|
| 小麦转录因子TaDL1生物信息学分析、亚细胞定位与表达分析 |
| ranscription Factor TaDL1 in Wheat Bioinformatics Analysis, Subcellular Localization, and Expression Analysis |
| 投稿时间：2024-02-03 修订日期：2024-04-30 |
| DOI： |
| 中文关键词: 小麦 TaDL1基因 生物信息学 亚细胞定位 表达分析 |
| 英文关键词:wheat TaDL1 gene bioinformatics subcellular localization expression analysis |
| 基金项目: |
|
| 摘要点击次数: 69 |
| 全文下载次数: 0 |
| 中文摘要: |
| YABBY转录因子家族是一类植物特有的转录因子,在植物叶片生长和花器官建成发挥重要作用,其N端具有C2C2型锌指结构域,C端有螺旋-环-螺旋YABBY结构域。本研究以春小麦品种新春9号为材料克隆到一个YABBY家族的TaDL1基因,编码区全长 603 bp,编码 201 个氨基酸。生物信息分析表明,TaDL1 蛋白分子量为22765.21Da,等电点为8.85,为不稳定亲水性蛋白；系统进化树和保守结构域分析表明,该基因属于YABBY家族的CRC亚家族成员,与水稻0SDL基因亲缘关系较近；转化烟草进行瞬时表达分析发现TaDL1蛋白定位在细胞核上。通过实时荧光定量分析组织特异性表达,发现TaDL1基因在小麦幼穗和叶片中特异性表达,其中在叶片中表达量最高,幼穗中次之。经过非生物胁迫发现该基因对盐和热胁迫响应,其中对盐胁迫响应最为明显且在24h内有持续较高的表达量,推测该基因可能参与盐胁迫应答相关的信号途径。该研究为后续深入探索TaDL1基因的生物学功能研究奠定了基础。 |
| 英文摘要: |
| The YABBY transcription factor family is a plant-specific group of transcription factors that play crucial roles in the growth of plant leaves and the development of floral organs. They possess a C2C2-type zinc finger domain at the N-terminus and a helix-loop-helix YABBY domain at the C-terminus. In this study, the TaDL1 gene of the YABBY family was cloned from the spring wheat variety Xin Chun 9. The coding region is 603 bp in length, encoding a protein of 201 amino acids. Bioinformatics analysis revealed that the TaDL1 protein has a molecular weight of 22765.21 Da, an isoelectric point of 8.85, and is an unstable hydrophilic protein. Phylogenetic tree and conserved domain analysis indicated that the gene belongs to the CRC subfamily of the YABBY family, with a close relationship to the rice OsDL gene. Transient expression analysis in transformed tobacco revealed nuclear localization of the TaDL1 protein. Real-time fluorescence quantitative analysis of tissue-specific expression showed that the TaDL1 gene exhibits specific expression in wheat spikelets and leaves, with the highest expression level in leaves and the second-highest in spikelets. Under abiotic stress, the gene responded significantly to salt and heat stress, with the most pronounced response to salt stress and sustained high expression within 24 hours. It is speculated that this gene may be involved in salt stress response-related signaling pathways. This study lays the foundation for further exploration of the biological functions of the TaDL1 gene. |
|
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
基本信息
