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| 贝莱斯芽胞杆菌LQ-3防治小麦纹枯病的效果及根际定殖能力 |
| Biocontrol Effect of Bacillus velezensis LQ-3 against Wheat Sharp Eyespot and Its Colonization Dynamics in the Rhizosphere of Wheat |
| 投稿时间：2023-12-03 修订日期：2024-03-27 |
| DOI： |
| 中文关键词: 贝莱斯芽胞杆菌LQ-3 小麦纹枯病 定殖 防效 |
| 英文关键词:Bacillus velezensis LQ-3 Wheat sharp eyespot Colonization Biocontrol effect |
| 基金项目:山西省基础研究计划(自由探索类)青年项目(20210302124679)；山西省现代农业产业技术体系建设专项资金资助(2023CYJSTX02-18)；农业基础性长期性科技工作农用微生物观测监测任务(NAES-AM-009)；农业基础性长期性科技工作植物保护监测(NAES-PP-017) |
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| 中文摘要: |
| 为明确贝莱斯芽胞杆菌(Bacillus velezensis)LQ-3防治小麦纹枯病的效果及其在小麦根部定殖能力,本研究利用LQ-3发酵液灌根处理小麦苗,调查统计LQ-3对小麦纹枯病的防效。同时利用绿色荧光蛋白标记菌株LQ-3(pGFP78)灌根处理小麦根部,并结合抗生素平板回收检测、激光共聚焦显微镜观察方法,探明LQ-3在小麦根部的定殖动态。结果表明,LQ-3防治小麦纹枯病的效果为57.49%,与化学药剂酷拉斯的防效59.95%相当。LQ-3(pGFP78)的生长趋势与野生型LQ-3相比并没有显著性差异,且仍具有游动性和生物膜形成能力。 LQ-3(pGFP78)发酵液初始浓度为2.3×10^8cfu/mL, 灌根处理5 d后,其在小麦根部的定殖量已达到9.63×10^6 cfu/g,25 d后,定殖量仍有1.33×10^6 cfu/g,表明LQ-3在小麦根部具有较好的定殖能力。该研究为贝莱斯芽胞杆菌LQ-3防治小麦纹枯病提供指导。 |
| 英文摘要: |
| To determine the biocontrol effect of Bacillus velezensis LQ-3 on wheat sharp eyespot and its colonization ability in wheat root, this study utilized LQ-3 fermentation to irrigate wheat root, aiming to investigate the biocontrol effect of LQ-3 against wheat eye spot. Additionally, the colonization dynamics of LQ-3 in wheat root were elucidated by using green fluorescent protein labeled strain LQ-3 (pGFP78) to irrigate wheat root, combined with antibiotic plate recovery detection and laser confocal microscopy observation. The results show that the biocontrol effect of LQ-3 on wheat sharp eyespot is 57.49%, which is equivalent to the biocontrol effect of the Kuras at 59.95%. Moreover, the growth trend of LQ-3 (pGFP78) exhibited no significant disparity when compared to the wild-type LQ-3, while still retaining its motility and biofilm-forming abilities. The initial concentration of LQ-3 (pGFP78) fermentation broth was 2.3×10^8cfu/mL, and after 5 days of root irrigation treatment, the colonization of LQ-3 (pGFP78) in wheat root had reached 9.63×10^6 cfu/g, even after 25 days, the colonization quantity still remain at 1.33×10^6 cfu/g, indicating that LQ-3 has proficient colonization capability in wheat root. The study provides a guidance for utilizing B. velezensis LQ-3 to control wheat sharp eyespot. |
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