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小麦种子萌发基因TaJAZ1的克隆和功能研究
Cloning and Functional Analysis of Seed Germination Related Gene TaJAZ1 in wheat(Triticum aestivum L.)
投稿时间:2022-12-05  修订日期:2023-07-17
DOI:
中文关键词:  小麦(Triticum aestivum L.)  萌发  TaJAZ1  ABA  基因功能验证
英文关键词:Wheat (Triticum aestivum L.)  Germination  TaJAZ1  ABA  Gene function verification
基金项目:陕西省重点研发计划项目(2022NY-172);陕西省“两链”融合专项“小麦种业创新工程项目”;杨凌种业创新重点研发计划(Ylzy-xm-03)
作者单位地址
姚雅鑫 西北农林科技大学农学院
农业农村部西北地区小麦生物学与遗传育种重点实验室 
陕西省杨凌示范区西北农林科技大学农学院
刘函西  
王伟伟  
张礼宁  
孙风丽  
张 超  
奚亚军* 西北农林科技大学农学院
农业农村部西北地区小麦生物学与遗传育种重点实验室 
陕西省杨凌示范区西北农林科技大学农学院
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中文摘要:
      铭贤169品种是我国黄淮麦区小麦育种广泛应用的条锈病诱发材料,但其种子休眠时间过长,不仅影响播种后均匀发芽和生长发育,其收获掉落籽粒也容易导致秋季育种田的生物学混杂。本研究通过筛选其休眠种子与萌发种子的转录组学数据,克隆获得差异表达基因TaJAZ1,并对其生物信息学特性、亚细胞定位、表达模式进行分析,结合解析拟南芥jaz3(与TaJAZ1同源性最高)突变体、TaJAZ1过表达拟南芥和水稻的表型反应。结果表明:TaJAZ1基因编码区全长1230bp,编码409个氨基酸,在不同物种间保守性较强,同野生二粒小麦JAZ1的亲缘关系最近;启动子区含有脱落酸响应元件ABRE 和茉莉酸甲酯响应元件CGTCA-motif和TGACG-motif;该基因定位于细胞核和细胞膜,TaJAZ1在种子发育中穗发芽时期表达量达到最高,进入成熟时期表达量显著降低;ABA能诱导TaJAZ1的表达,ABA处理下过表达拟南芥较野生型和jaz3突变体萌发率更高,ABA信号通路基因AtABI5的诱导量变低;TaJAZ1过表达水稻的萌发率高于受体水稻, ABA处理后,OsABI5的诱导量也降低。以上结果证明了TaJAZ1能促进种子萌发,进一步验证其在ABA信号通路中起负调控作用,为改良铭贤169等休眠性强的小麦品种提供了参考。
英文摘要:
      Mingxian 169 is a stripe rust inducing material widely used in wheat breeding in Huanghuai wheat region of China. However, its seed dormancy time is too long, which not only affects the uniform germination, growth and development after sowing, but also easily leads to biological promiscuity in autumn breeding field when the seed is harvested and dropped. In this study, the differentially expressed gene TaJAZ1 was cloned and obtained by screening the transcriptomics data of its dormant and germinated seeds, and its bioinformatics characteristics, subcellular localization and expression pattern were analyzed, together with the phenotypic responses of Arabidopsis jaz3 mutant (with the highest homology with TaJAZ1), TaJAZ1 overexpressing Arabidopsis and rice. The results showed that the coding region of TaJAZ1 gene was 1230bp in length, encoding 409 amino acids, which was highly conservative among different species and had the closest genetic relationship with wild two-grain wheat JAZ1. The promoter region contained ABA-responsive element ABRE and methyl jasmonate-responsive elements CGTCA-motif and TGACG-motif; The gene was located in the nucleus and cell membrane. The expression level of TaJAZ1 reached the highest in the pre-harvest sprouting stage of seed development and significantly decreased in the maturation stage. ABA could induce the expression of TaJAZ1. Compared with the wild-type Arabidopsis and jaz3 mutants, the overexpressed Arabidopsis under ABA treatment exhibited higher germination rate, and the induction amount of ABA signaling pathway gene AtABI5 was decreased. The germination rate of rice overexpressed by TaJAZ1 was higher than that of the receptor rice, and the induction of OsABI5 was also reduced after ABA treatment. The above results proved that TaJAZ1 could promote seed germination and further verified its negative regulatory role in ABA signaling pathway, which provided a reference for improving strong dormancy wheat varieties such as Mingxian 169.
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