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麦蚜及植物病毒胁迫下小麦体内保护酶和解毒酶活性变化
Activities of Protective and Detoxification Enzymes in Wheat under Exposure of Aphids and Plant Viruses
投稿时间:2019-08-19  修订日期:2019-11-12
DOI:
中文关键词:  小麦  大麦黄矮病毒  麦蚜  保护酶  解毒酶
英文关键词:wheat  Barley yellow dwarf virus  wheat aphid  defense enzyme  detoxifying enzyme
基金项目:国家自然科学基金(31401735);中央高校基本科研业务费专项资金(2452017060)
作者单位E-mail
张 弛 旱区作物逆境生物学国家重点实验室西北农林科技大学植物保护学院 zhangchizc@nwafu.edu.cn 
孟琳钦   
苏 丹   
仝则乾   
胡祖庆 旱区作物逆境生物学国家重点实验室西北农林科技大学植物保护学院 huzuqing@nwsuaf.edu.cn 
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中文摘要:
      为探究蚜虫及植物病毒胁迫下小麦体内酶活性的变化规律,为研究蚜虫-病毒-小麦三者互作关系奠定基础。本研究以麦二叉蚜Schizaphis graminum、禾谷缢管蚜Rhopalosiphum padi和麦长管蚜Sitobion avenae为试虫,以大麦黄矮病毒(Barley yellow dwarf virus,BYDV)的GAV株系为测试病毒,研究蚜虫、植物病毒及两者共同胁迫下小麦体内超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)和过氧化物酶(POD)等保护酶及酸性磷酸酶(ACP)和碱性磷酸酶(AKP)等解毒酶的活性变化。试验结果表明:与健康小麦相比,麦二叉蚜胁迫下小麦体内ACP活性显著升高,升高100.46%,禾谷缢管蚜胁迫下小麦体内POD活性显著降低,降低34.59%,麦长管蚜胁迫下小麦体内POD、CAT、SOD、ACP和AKP均显著升高,分别升高83.88%,126.45%,91.21%,197.26%,468.82%。与未感染病毒小麦相比,BYDV-GAV胁迫下,小麦体内SOD活性显著升高,升高37.41%。小麦感染BYDV-GAV后,与未受蚜虫胁迫对照小麦相比,麦二叉蚜胁迫下小麦体内POD、CAT和ACP活性显著升高,分别升高73,66%,110.72%,50.49%,禾谷缢管蚜胁迫下小麦体内CAT和ACP活性显著上升,分别上升115.13%,37.00%,麦长管蚜胁迫下小麦体内POD、CAT和AKP均显著升高,分别升高97.58%,70.19%,3665.03%。分析表明,麦二叉蚜、麦长管蚜和BYDV-GAV胁迫下均能引起小麦体内酶活的显著上升,而禾谷缢管蚜胁迫下能引起小麦体内酶活的显著下降。麦二叉蚜、禾谷缢管蚜和麦长管蚜胁迫均能引起感染BYDV-GAV小麦体内酶活的显著上升。说明麦蚜和植物病毒均能改变小麦体内生理生化进程,但效应有差异。
英文摘要:
      In order to explore the change rule of enzyme activities in wheat under the stress of aphid and plant virus, which laid a foundation for studying the interaction between aphids, viruses and wheat. We used the wheat aphid Schizaphis graminum, Rhopalosiphum padi and Sitobion avenae as test insects, the GAV strain of Barley yellow dwarf virus (BYDV) as test virus to study the activity changes of oxidative enzymes (including peroxidase POD, catalase CAT, superoxide dismutase SOD) and detoxifying enzymes (including acid phosphatase ACP, alkaline phosphatase AKP),which under aphids, plant viruses and both common stress in wheat. Experimental results show that compared with healthy wheat, the rangeability of enzyme activities in wheat was significant, activity of ACP in wheat increased by 100.46% under the stress of S. graminum, the activity of POD in wheat decreased by 34.59% under the stress of R. padi, and the activity of POD, CAT, SOD, ACP and AKP increased respectively by 83.88%, 126.45%, 91.21%, 197.26% and 468.82% under the stress of S. avenae. Compared with virus-uninfected wheat, the activity of SOD in wheat increased significantly by 37.41% under the stress of BYDV-GAV. After BYDV-GAV infection in wheat, compared with untreated wheat, the activity of POD, CAT and ACP increased significantly by 73,66%, 110.72% and 50.49% under the stress of S. graminum, the activities of CAT and ACP increased significantly by 115.13% and 37.00% under the stress of R. padi, and under the stress of S. avenae, the activities of POD, CAT and AKP increased significantly by 97.58%,70.19% and 3665.03%. The analysis demonstrated that under the stress of S. graminum, S. avenae and BYDV-GAV, the enzyme activity in wheat could be significantly increased, and the stress of R. padi could cause a significant decrease. The stress of S. graminum, R. padi and S. avenae can cause a significant increase in the enzyme activity of BYDV-GAV infected wheat. It indicated that both wheat aphid and plant virus can change the physiological and biochemical processes with different effects in wheat.
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