| 吴国丽，魏霁桐，段江丽，盛呈泽，宋齐鲁，郭佳林，张亚敏，牛 娜，王军卫，马守才，张改生，宋瑜龙.小麦 TaWRKY51基因的克隆及其参与SQ-1诱导小麦花粉败育过程的表达模式研究[J].麦类作物学报,2021,(12):1443 |
| 小麦 TaWRKY51基因的克隆及其参与SQ-1诱导小麦花粉败育过程的表达模式研究 |
| Cloning of TaWRKY51 and Analysis of Its Expression Pattern during the Development of Pollen Abortion in Male Sterility Line Induced by SQ-1 in Wheat(Triticum aestivum) |
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| DOI：10.7606/j.issn.1009-1041.2021.12.01 |
| 中文关键词: 小麦 TaWRKY51 亚细胞定位 表达分析 |
| 英文关键词:Wheat TaWRKY51 Subcellular localization Expression analysis |
| 基金项目:国家自然科学基金项目（31701500）；中国博士后科学基金项目（2017M613222）；中央高校基本科研业务费专项资金项目（2452017056） |
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| 中文摘要: |
| 为进一步深入解析化学杂交剂SQ-1诱导小麦花粉败育的机理，本研究从SQ-1诱导的生理型雄性不育系PHYMS-1376及其对照可育系CK-1376各时期花药转录组测序筛选出差异表达基因TraesCS1D02G362900.1，与小麦基因组数据库比对发现，该基因编码的氨基酸与 TaWRKY51同源度为97%，说明该差异表达基因为 TaWRKY51对其进行生物信息学分析、亚细胞定位、转录激活活性与花药表达模式研究，结果表明， TaWRKY51开放阅读框全长663 bp，可编码220个氨基酸，含有WRKY结构域和锌指结构，分子量约为23.34 kD，等电点为6.42；启动子区包含与光响应、茉莉酸甲酯响应、脱落酸响应等7类顺式作用元件，且TaWRKY51蛋白被定位在细胞核内，具有一定的转录激活活性；与CK-1376相比，PHYMS-1376植株花药中 TaWRKY51的表达量在5个发育时期均高于CK-1376，且在四分体时期、单核晚期、二核期和三核期与CK-1376的差异均达极显著水平，表明该基因与PHYMS-1376花粉败育密切相关。 |
| 英文摘要: |
| In order to elucidate the molecular mechanism of pollen abortion induced by the chemical hybrid agent SQ-1 in wheat,TraesCS1D02G362900.1 was screened out from the significantly different expression genes based on transcriptme sequencing of PHYMS-1376 with pollen abortion in male sterility induced by SQ-1 and its control fertile line CK-1376.Compared with wheat genome database,found that the similarity was 97% between the protein encoded by this gene and TaWRKY51,so the differentially expressed gene TraesCS1D02G362900 was TaWRKY51. TaWRKY51 was analysed using bioinformatics technology,transcriptional activation activity,sub-cellular localization,and the anther tissue expression pattern of TaWRKY51 was also performed. The results showed that TaWRKY51 containing an open reading frame with 663 bp and encoding 220 amino acids with a WRKY domain and zinc finger structure. Meanwhile,TaWRKY51 protein was a mono-mer protein with molecular weight of 23.34 kD and isoelectric point of 6.42.Cis-acting elements 7 categeries were found to be distributed in promoter regions, related to light responsiveness,MeJA-responsiveness,abscisic acid responsiveness and so on. TaWRKY51 was located in cell nucleus with certain transcriptional activation.Compared with CK-1376, the expression level of TaWRKY51 was higher at five development stages in anther of PHYMS-1376, with significant difference at tetrad stage, late uninucleate stage, binucleate stage and trinucleate stage, indicating that TaWRKY51 maybe closely associated with pollen abortion in anther of PHYMS-1376. |
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