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刘 娟,张 凯,范胜男,韩 洋,冯玉梅,李星岩,韩 冰,杨 燕.小麦矮秆基因 Rht-B1b Rht-D1b的检测及其验证[J].麦类作物学报,2021,(7):816
小麦矮秆基因 Rht-B1b Rht-D1b的检测及其验证
Molecular Detection and Verification of Dwarfing Gene Rht-B1b and Rht-D1b in Wheat
  
DOI:10.7606/j.issn.1009-1041.2021.07.04
中文关键词:  小麦  分子标记  矮秆基因  株高
英文关键词:Wheat  Molecular marker  Dwarfing gene  Plant height
基金项目:国家自然科学基金项目(31960424)
作者单位
刘 娟,张 凯,范胜男,韩 洋,冯玉梅,李星岩,韩 冰,杨 燕 (内蒙古农业大学生命科学学院植物生物技术功能实验室/内蒙古自治区植物逆境生理与分子生物学重点实验室内蒙古呼和浩特 010018) 
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中文摘要:
      为研究矮秆基因 Rht-B1b Rht-D1b在小麦品种中的分布及其对株高的影响,以3个小麦群体(中国冬麦区白粒小麦品种、中国小麦历史品种、春小麦育种材料)共321份小麦品种为材料,检测矮秆基因 Rht-B1b Rht-D1b的分布频率,并分析比较它们对春小麦育种材料株高的影响。结果表明,在检测的321份材料中,共有193份材料含有 Rht-B1b矮秆基因,分布频率为60.1%,其中,中国冬麦区白粒小麦品种、中国小麦历史品种、春小麦育种材料中含有 Rht-B1b矮秆基因的材料分别有29、77和87份,分布频率分别为 34.5%、72.6%和66.4%;共有135份材料含有 Rht-D1b矮秆基因,分布频率为42.1%,其中,中国冬麦区白粒小麦品种、中国小麦历史品种、春小麦育种材料中含有 Rht-D1b 矮秆基因的材料分别有41、21和73份,分布频率分别为48.8%、19.8%和55.7%;共有78份材料同时含有 Rht-B1b Rht-D1b矮秆基因,分布频率为 24.3%,其中,中国冬麦区白粒小麦品种、中国小麦历史品种、春小麦育种材料中同时含有 Rht-B1b Rht-D1b矮秆基因的材料分别有15、17和46份,分布频率分别为17.6%、15.9%和35.1%。对已被鉴定株高表型的91份春小麦育种材料进一步分析发现,同时携带 Rht-B1b Rht-D1b矮秆基因的材料株高较低。
英文摘要:
      In order to study the distribution of dwarfing genes Rht-B1b and Rht-D1b in wheat and their effects on plant height,we detected the distribution of Rht-B1b and Rht-D1b genes in 321 wheat varieties from three populations(white-grain wheat cultivars in China winter wheat areas,historical wheat cultivars and natural spring wheat population), and analyzed the effect of dwarfing genes Rht-B1b and Rht-D1b on plant height of spring wheat breeding materials.The results showed that among 321 materials,193 materials contained Rht-B1b gene with the frequency of 60.1%, and among them there are 29,77 and 87 materials contained Rht-B1b gene in white-grain wheat cultivars from China winter wheat areas,historical wheat cultivars and spring wheat breeding materials,with the frequency of 34.5%,72.6% and 66.4%, respectively; 135 materials contained Rht-D1b gene with the frequency of 42.1%, and among them there are 41, 21 and 73 materials contained Rht-D1b gene in white-grain wheat cultivars from China winter wheat areas,historical wheat cultivars and spring wheat breeding materials, with the frequency of 48.8%, 19.8% and 55.7%,respectively. In addition, 78 of the 321 materials contained both Rht-B1b and Rht-D1b genes with the frequency of 24.3%, and among them there are 15, 17 and 46 materials contained Rht-B1b and Rht-D1b genotype in white-grain wheat cultivars from China winter wheat areas,historical wheat cultivars and spring wheat breeding materials, with the frequency of 17.6%, 15.9% and 35.1%, respectively. Further analysis found that materials with both Rht-B1b and Rht-D1b genes have lower plant heights among the 91 materials with plant height data.
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