| In order to clarify the resistance mechanism of Zhong 4 to stripe rust, transcriptome sequencing was used, and the differentially expressed genes produced at 12, 24 and 48 h after inoculation of strip rust were analysed, using those at 0 h as the control. The results showed that 5 151 up-regulated differentially expressed genes(DEGs) were obtained, including 4 482 localized genes and 669 new genes(the location of its chromosomes cannot be determined). Among these, locations on A, B, and D chromosomes accounted for 30.75%, 35.27%and 33.98%, respectively. GO function annotation classified these DEGs into 46 functional groups of three major classes, mainly involving catalytic activity, transporter activity, cellpart,cell, organelle, metabolic process, cellular process, single-organism process, etc. KEGG pathway analysis showed that 4 482 localized DEGs were mostly related to spliceosome, phenylalanine metabolism, peroxisome, galactose and amino acid metabolism, etc; New genes were mostly related to sugar synthesis and metabolism, amino acid metabolism, etc.33 DEGS were found in plant-pathogenic interaction, encoding disease resistance proteins, MLO proteins, NAC and WRKY transcription factors, and so on. The results laid a foundation for further analysis on resistance mechanisms to stripe rust in Zhong 4. |
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