| In order to elucidate the molecular mechanism of pollen abortion induced by chemical hybridization agent SQ-1 in wheat,the differential expression genes were screened by transcriptomic profiling in anthers of CK-1376 and PHYMS-1376 at tetrad,early uninucleate,late uninucleate,binucleate and trinucleate stages,respectively. Meanwhile,these differential expression genes were annotated using GO enrichment,KEGG pathway enrichment and GSEA enrichinent.The ABA and JA contents were measured in anthers of CK-1376 and PHYMS-1376 at the above five stages using high-performance liquid chromatography. The expression pattern of the key genes involved in metabolic pathway of ABA and JA were analysed by qRT-PCR.The results showed that:compared with CK-1376,a total of 36 058 differential expression genes were screened in anthers of PHYMS-1376 at these five stages,mainly enriched in plant hormone signal transduction,phenylpropanoid biosynthesis,starch and sucrose metabolism,and amino acid biosynthesis pathway. In comparison,the JA content of anther was significantly(P<0.05) lower at tetrad stage,increased significantly(P<0.05) at early uninucleate stage,and decreased significantly(P<0.05) at late uninucleate stage in PHYMS-1376,whereas JA content of anther was significantly(P<0.05) higher at binucleate stage,decreased sharply at trinucleate stage in PHYMS-1376.The ABA content of anther was higher from the tetrad to trinucleate stage in PHYMS-1376,reaching to asignificant level(P<0.05) at early uninucleate,late uninucleate and binucleate stages,which may be attributed to the key gene(TraesCS1D02G271000) involved PP2C protein with lower expression level,leading to the reduction of the PP2C protein content,activating BIN2/BILs kinase pathway,causing SnRK2s phosphorylation and thus increasing ABA level. These results indicated that a comparatively large fluctuation of JA contents,increasing level of ABA in anthers of PHYMs-1376 at five stages,along with the differential expression of key genes in JA and ABA pathways may be closely related to pollen abortion induced by the chemical hybridization agent SQ-1. This study could provide a theoretical basis for further revealing the mechanism of pollen abortion induced by SQ-1 in wheat. |
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