| 刘朦朦，张萌娜， 张倩倩，刘锡建，郭宇航，孙靳惠，武亚瑞，王素容，吴永振，孙 晗，崔 法，赵春华.小麦旗叶宽主效QTL QFlw-5B遗传效应解析[J].麦类作物学报,2019,(12):1399 |
| 小麦旗叶宽主效QTL QFlw-5B遗传效应解析 |
| Genetic Analysis of a Major Stable QTL QFlw-5B for Wheat Flag Leaf Width |
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| DOI：10.7606/j.issn.1009-1041.2019.12.02 |
| 中文关键词: 小麦 旗叶宽 主效QTL QFlw-5B 初级定位 遗传解析 |
| 英文关键词:Wheat Flag leaf width Major QTL QFlw-5B Primary QTL mapping Genetic analysis |
| 基金项目:国家级大学生创新创业计划项目(201710451344，201710451349， 201810451050); 植物细胞与染色体工程国家重点实验室开放课题(PCCE-KF-2017-06); 鲁东大学创新课题立项(ld1811,ld1812,ld1813,ld1843,ld1844) |
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| 中文摘要: |
| 为了明确位于小麦5B染色体上的一个旗叶宽主效QTL QFlw-5B的遗传效应，对5B染色体进一步加密，从而缩小靶区段的范围，在加密图谱的基础上，利用衍生自科农9204×京411的188个重组自交系群体（KJ-RIL），对8个环境下旗叶宽做进一步的定位分析，将QFlw-5B定位于AX-110978403~AX-111671812的61.22~68.60 cM遗传距离范围内，能够解释9.40%~19.76%的旗叶宽表型变异，来自科农 9204的等位基因增加旗叶宽0.04~0.07 cm。利用与QFlw-5B紧密连锁标记AX-108884656对188个 KJ-RIL家系进行遗传分析，结果表明，QFlw-5B优异等位基因在 8个环境下均能增加穗粒数，在 6个环境下能增加千粒重和单株产量，而对单株穗数有一定的负效应。利用 310份育成品种(系)对QFlw-5B优异单倍型的应用情况进行分析，结果表明，QFlw-5B优异单倍型虽然已经被育种家选择，但还有较大的遗传改良空间。 |
| 英文摘要: |
| In order to determine the genetic effect of a flag leaf width major effect QTL QFlw-5B on wheat chromosome 5B,chromosome 5B was further enriched to narrow the range of the target region. Based on the increased density map,the 188 KJ-RILs population derived from a cross between Kenong 9204 and Jing 411 in eight different environments were used for QTL mapping,and QFlw-5B was mapped between AX-110978403 and AX-111671812 with the genetic distance from 61.22 cM to 68.60 cM,which explained 9.40% to 19.76% of the phenotypic variation. The alleles from Kenong 9204 increased flag leaf width(FLW) with 0.04-0.07 cm. AX-10884656,a molecular marker closed linkage with QFlw-5B ,was used for genetic analysis in the 188 KJ-RIL families,and the results showed that excellent alleles of QFlw-5B increased kernel number per spike(KNPS) in the eight environments. Meanwhile,it increased thousand-kernel weight(TKW) and grain yield per plant(GYPP) in the six environments,while it had negative effect on spike number per plant(SNPP). The application of QFlw-5B haplotype was analyzed with 310 bred varieties(lines),and the results showed that the excellent haplotype of QFlw-5B has been utilized in wheat breeding programs,while there was still great potential in genetic improvement of wheat. |
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