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满君霞,张国华,徐加利,吴盼盼,韩 旭,赵 岩,李斯深.小麦RIL群体苗期抗旱性状的QTL分析[J].麦类作物学报,2019,(8):912
小麦RIL群体苗期抗旱性状的QTL分析
QTL Mapping for Drought Resistance Related Traits in Wheat Using RIL Population at Seedling Stage
  
DOI:10.7606/j.issn.1009-1041.2019.08.04
中文关键词:  小麦  重组自交系  抗旱  QTL
英文关键词:Wheat  Recombinant inbred line(RIL)  Drought resistance  Quantitative trait locus(QTL)
基金项目:“十三五”国家重点研发计划“七大作物育种”重点专项(2017YFD0100600);山东省现代农业产业技术体系项目
作者单位
满君霞,张国华,徐加利,吴盼盼,韩 旭,赵 岩,李斯深 (1.山东农业大学农学院/作物生物学国家重点实验室山东泰安 2710182.泰安市农业局山东泰安 271000 3.滨州市农业局山东滨州 256600) 
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中文摘要:
      为给小麦抗旱基因克隆以及分子标记辅助育种提供参考,以小麦“泰农18×临麦6号”RIL群体的184个家系为材料,用PEG-6000模拟干旱胁迫对小麦苗期抗旱相关性状进行QTL分析。结果,共检测到43个QTL,位于除1B、3D、4D、5A、5D、6D和7A外的14条染色体上,其中,控制根数的QTL 9个、苗高的QTL 5个、最大根长的QTL 5个、苗鲜重的QTL 3个、根鲜重的QTL 6个、苗干重的QTL 1个、根干重的QTL 3个、鲜重根冠比的QTL 3个、干重根冠比的QTL 3个,控制抗旱系数的QTL为5个。单一QTL可解释3.39%~32.63%的表型变异。28个QTL为正值,表明QTL的增加效应来自于母本泰农18;30个QTL表现为负值,表明其增加效应来自于父本临麦6号。11个QTL为在两个或两个以上环境下检测到的相对高频QTL(RHF-QTL)。在4B染色体上检测到1个QTL簇,包括4个形态性状(苗高、苗鲜重、苗干重、鲜重根冠比)的RHF-QTL( QSh-4B-1 QSfw-4B-1 QSdw-4B-1 QRsfw-4B-1)和1个抗旱系数QTL( QRsfw-D-4B-1),其贡献率均超过10%。该QTL簇的分子标记可以用于标记辅助选择。
英文摘要:
      In order to provide reference for drought resistance gene cloning and marker assisted selection,QTL(quantitative trait locus) analysis was carried out in wheat under PEG-6000 treatment using 184 RILs(recombinant inbred lines) derived from a cross of Tainong 18×Linmai 6.The results showed that a total of 43 QTLs were located on 14 chromosomes except for 1B,3D,4D,5A,5D,6D and 7A.Of these,5,5,9,3,6,1,3,3 and 3 QTLs were related to shoot hight,maximum root length,root number,shoot fresh weight,root fresh weight,shoot dry weight,root dry weight,root-shoot ratio in fresh weight,root-shoot ratio in dry weight,respectively; and 5 QTLs were related to drought resistance coefficient.A single QTL accounted for 3.39 to 32.63% of the phenotypic variation.Twenty-eight QTLs showed positive additive effects,indicating that Tainong 18 increased the effects of QTLs; whereas 30 QTLs showed negative additive effects,indicating that Linmai 6 increased the effects of QTLs.A number of 11 RHF-QTLs( relative high frequency QTLs) were detected in two or more environments.One QTL cluster was detected on chromosome 4B,including four morphological traits(shoot hight,shoot fresh weight,shoot dry weight,and root-shoot ratio in fresh weight) of RHF-QTLs( QSh-4B-1, QSfw-4B-1, QSdw-4B-1, QRsfw-4B-1) and one drought resistance coefficient QTL( QRsfw-D-4B-1).The contribution rates of the five QTLs were all over 10%.The markers of this QTL cluster can be used for marker assisted selection.
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