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作者	单位
丁明亮，赵佳佳，周国雁，李宏生，崔永祯，赵红，伍少云，杨木军，郑军，李绍祥	(1.云南省农业科学院粮食作物研究所,云南昆明 650205；2.山西省农业科学院小麦研究所,山西临汾 041000；3.云南省农业科学院生物技术与种质资源研究所，云南昆明 650205)

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中文摘要:

为深入了解云南省建国以来普通小麦育成品种（系）的高分子量麦谷蛋白亚基(HMW-GS)组成情况，利用SDS-PAGE电泳技术对152份云南省1950s以来普通小麦育成品种（系）HMW-GS组成和变异进行了分析。结果表明：（1）云南省普通小麦在Glu-A1位点具有N（56.58%）和1（43.42%）2种亚基类型,在Glu-B1位点具有7+8（42.11%）、7+9（34.87%）、6+8（0.66%）、14+15（7.24%）、17+18（13.16%）和13+16（1.97%）6种亚基类型,在Glu-D1位点具有2+10（5.26%）、2+12（54.61%）、5+10（24.34%）和5+12（15.79%）4种亚基类型；（2）云南省普通小麦HMW-GS组合类型比较丰富,共出现27种亚基组合类型,其中“N，7+8，2+12”、“N，7+9，2+12”、“1，7+8，2+12”与“ 1，7+9，5+10”较多，出现频率分别为20.39%、9.87% 、7.89%和7.89%；（3）云南省各个时期育成品种（系）的HMW-GS品质评分基本维持在4.50左右，1990s以后育成的品种（系）中优质亚基5+10出现的频率随普通小麦育成时期的推移而逐渐增加。由此可见，云南省普通小麦的HMW-GS在 Glu-A1、Glu-B1、Glu-D1位点上表现出丰富的多态性，共有 12种HMW-GS等位变异，包括13+16、2+10和5+12三种稀有亚基类型和27种亚基组合类型；对加工品质具有正效应的优质亚基17+ 18和 5+ 10频率较小，缺乏优质亚基2*。因此，在云南省普通小麦的品质改良中应加强优质亚基2*、17+18和 5+10引入及合理应用。

英文摘要:

In this paper,we detected the composition of high molecular weight glutenin subunits(HMW-GS) of wheat varieties/lines from Yunnan Province since 1950s,and Provide evidence for wheat quality breeding in Yunnan Province. The compositions and variation of HMW-GS controlled by the Glu-1 of 152 inbred varieties and lines in Yunnan Province were analyzed using the method of sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE).Results showed that:two allelic variations,the subunits N and 1,with frequencies of 56.58% and 43.42%,respectively,were detected at Glu-A1; six allelic variations,the subunits 7+8,7+9,6+8,14+15,17+18 and 13+16,with frequencies of 42.11%,34.87%,0.66%,7.24%,13.16% and 1.97%,respectively,were detected at Glu-B1; four allelic variations,the subunits 2+10,2+12,5+10 and 5+12,with frequencies of 5.26% ,54.61%,24.34% and 15.79%,respectively,were detected at Glu-D1. A total of 27 combination types were observed in inbred wheat varieties/lines of Yunnan Province. Among them,‘N,7+8,2+12’,‘N,7+9,2+12’,‘1,7+8,2+12’ and ‘1,7+9,5+10’ appear more frequent,with frequencies of 20.39%,9.87%,7.89% and 7.89%,respectively. The HMW-GS subunit quality score of all varieties/lines in Yunnan was around 4.50. The frequency of good quality subunit 5+10 increased gradually with the breeding period in bred varieties and lines after 1990s. A fairly high level of genetic variation at Glu-1 locus was observed,12 different alleles,including three rare compositions 13+16,2+10 and 5+12,were identified at the Glu-A1,Glu-B1 and Glu-D1 loci,respectively,and 27 combination types were observed in inbred wheat varieties and lines of Yunnan Province. The good quality subunits 17+18 and 5+10,which have positive effects on processing quality,have less frequency,and there is a lack of good quality subunit 2* at the same time. Therefore,the introduction and rational application of high quality subunits 2*,17+18 and 5+10 should be strengthened in the quality improvement of common wheat breeding in Yunnan province.

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