| In order to explore more QTLs that control flag leaf size and spike related traits in wheat,a population of 133 F6：7 recombinant inbred lines(RILs) derived from a cross between Lankao 906 and Xiaoyan 81 was used to detect the QTLs for flag leaf size and spike related traits in six environments. The results showed that 202 SSR markers were used to construct genetic linkage maps,covering 21 wheat chromosomes,with the full mapping length of 1 678.93 cM and an average genetic distance of 8.30 cM between the markers. A total of 30 QTLs were detected on 1B,2A,3D,4A,4B,4D,5D,6A,6B,6D and 7D chromosomes by Inclusive Composite Interval Mapping(ICIM,LOD>2.5). There were seven QTLs for flag leaf width(FLW),nine QTLs for spike length(SL),five QTLs for spikelets per spike(SPS),five QTLs for kernels per spike(KER),and four QTLs for spike compactness(SC),with the phenotypic variations ranging from 4.94% to 23.14% in different environments. Among these QTLs, Qflw-4A was detected in three environments with phenotypic variations ranging from 10.13% to 20.77%,which was a stable and major QTL associated with FLW. Qsl-4D.2 was detected in four environments with phenotypic variations ranging from 12.58% to 23.14%,which was a stable and major QTL associated with SL. Qker-5D was detected in two environments with phenotypic variations ranging from 11.44% to 14.32%,which was a stable and major QTL associated with KER. The three stable and major QTLs on chromosomes 4A,4D and 5D identified in this study might be useful for marker-assisted selection of flag leaf size and spike traits in wheat breeding. |
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