| Drought may be one of the most important abiotic stresses limiting crop growth and yield. It was reported that more than 50% of the wheat planting area is affected by periodic drought in recent years,and drought has become a main limiting factor of wheat production. Here,we report the drought tolerance of a transgenic wheat line G19-349 containing GmDREB1 gene. The transgenic wheat line G19-349 and its receptor parent Jimai 19 were used as materials. Relative chlorophyll content,gas exchange and chlorophyll fluorescence parameters,the activity of antioxidant enzyme including superoxide dismutase(SOD) peroxidase(POD) and catalase(CAT),the content of malondialdehyde(MDA),and grain yield were determined. The results showed that G19-349 had higher SPAD value,net photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductance(Gs),intercellular CO2 concentration(Ci),maximum photochemical efficiency(Fv/Fm),actual photochemical efficiency of photosystem Ⅱ(ФPSⅡ) the activity of SOD,POD and CAT,and grain yield,but lower content of MDA than those of Jimai 19 under treatment with twice irrigation. While these indicators showed no significant difference between G19-349 and Jimai 19,under treatment with four irrigation. Compared with Jimai 19,G19-349 exhibited lower water consumption,but higher water use efficiency and grain yield,under treatment with three irrigation. The results indicate that G19-349 had higher chlorophyll content and activity of antioxidant enzyme than Jimai19,which can alleviate membrane lipid peroxidatione,maintain relatively higher photosynthetic efficiency,and ultimately its drought tolerance was improved. |
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