| 王化敦，高春蕾，张 鹏，张 瑜，张平平，马鸿翔.长江中下游麦区小麦品种籽粒硬度及puroindoline基因等位变异的分子检测[J].麦类作物学报,2017,(4):438 |
| 长江中下游麦区小麦品种籽粒硬度及puroindoline基因等位变异的分子检测 |
| Allelic Variation of puroindoline Alleles in Bread Wheats from Lower-Middle Reaches of the Yangtze River |
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| DOI：10.7606/j.issn.1009-1041.2017.04.02 |
| 中文关键词: 普通小麦 籽粒硬度 puroindoline基因 |
| 英文关键词:Bread wheat Grain hardness puroindoline alleles |
| 基金项目:国家自然科学基金项目(31501819)；国家现代农业产业技术体系项目(CARS-3)；江苏省自然科学基金项目(BK20161375) |
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| 中文摘要: |
| 为了明确长江中下游麦区小麦籽粒硬度及puroindoline基因型的分布，以该麦区105份小麦育成品种为材料，利用单籽粒硬度仪(SKCS)测定其籽粒硬度，利用分子标记检测和基因序列分析鉴定puroindoline基因的等位变异。结果表明，在长江中下游麦区历年育成的小麦品种中软质麦比例较高，占52.4%，硬质麦和混合麦分别占38.1%和9.5%；硬质麦和混合麦中存在 Pinb-D1b、 Pina-D1b和 Pinb-D1p三种变异类型，突变频率分别为29.5%、10.5%和3.8%。 |
| 英文摘要: |
| To elucidate the grain hardness index and the distribution of hardness-related gene in the lower-middle reaches of the Yangtze River wheat region, 105 wheat varieties widely collected from this region were used in this study. Grain hardness was examined by a Single Kernel Characterization System (SKCS). Molecular detection and sequencing analysis were used to identify puroindoline alleles. The results showed that soft wheat accounts for 52.4%, while hard and mixed wheat account for 38.1% and 9.5%, respectively. Three mutant puroindoline alleles of Pinb-D1b, Pina-D1b and Pinb-D1p were detected in hard and mixed wheat. Of them, the frequency of Pinb-D1b was the highest, with the percentage of 29.5%, followed by Pina-D1b and Pinb-D1p, with the percentage of 10.5% and 3.8%, respectively. |
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