| 王建武，何心尧，王 辉，夏先春，何中虎.S组山羊草属 Psy1基因片段的克隆与序列分析[J].麦类作物学报,2017,(4):429 |
| S组山羊草属 Psy1基因片段的克隆与序列分析 |
| Cloning and Sequence Analysis of Phytoene Synthase 1 ( Psy1) Gene Fragment in Aegilops Species of S Genome |
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| DOI：10.7606/j.issn.1009-1041.2017.04.01 |
| 中文关键词: 小麦籽粒黄色素 山羊草 八氢番茄红素合成酶基因 Psy1 基因片段克隆 |
| 英文关键词:Yellow pigment of wheat grain Aegilops Phytoene synthase 1 gene( Psy1) Cloning of gene fragment |
| 基金项目:长江大学博士科研启动基金项目（118/801180010115） |
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| 中文摘要: |
| 类胡萝卜素含量是影响小麦面制品色泽的主要因素，而 Psy1基因则是类胡萝卜素生物合成中的关键基因。为了了解小麦近缘种S组山羊草的 Psy1基因多样性分布规律，以高大山羊草、双角山羊草及西尔斯山羊草为材料，应用同源基因克隆技术对其进行 Psy1基因克隆。结果表明，利用分子标记YP7B-5检测获得的8个新 Psy1-S1等位变异的部分序列覆盖了 Psy1基因的第二外显子和第二内含子的全部以及第三外显子93.1%的区域。在与包括来自普通小麦的 Psy1-B1d、栽培二粒小麦的 Psy1-B1k以及拟斯卑尔脱山羊草的 Psy1-S1a、 Psy1-S1b、 Psy1-S1c对应部分的序列综合分析发现一致性高达93.4%~98.5%，而且编码区序列差异均为同义突变，没有造成编码氨基酸的变化。而第二内含子序列则存在数个SNP及InDel，尤其是 Psy1-S1h的161 bp大片段插入和 Psy1-S1i的177 bp大片段缺失，导致与其他 Psy1-B1/ Psy1-S1等位变异产生较大的长度差异。分析表明，内含子的差异是造成 Psy1基因多样性的主要原因。 |
| 英文摘要: |
| Carotenoids content(YPC) is the main factor influencing wheat flour color. The phytoene synthase 1( Psy1) gene encodes a key enzyme to determine the carotenoids content of endosperm in wheat.Aegilops species are important resources for wheat genetic improvement. In the present study,three Aegilops species including Ae.longissima(SS),Ae.bicornis(SS) and Ae.searsii(SS) were used to clone Psy1 genes in order to disclose their genetic diversity. Using homologous gene cloning approach,the partial Psy1 gene sequences of eight novel allelic variants at Psy1-S1 locus were obtained by the marker YP7B-5,covering the whole region of the second exon and the second intron and the 93.1% region of the third exon. High sequence similarity of 93.4% to 98.5% was shown between these eight Psy1-S1 alleles and other five Psy1-B1/ Psy1-S1 alleles,including Psy1-B1d from common wheat, Psy1-B1k from T.dicoccum and Psy1-S1a, Psy1-S1b, Psy1-S1c from Ae.speltoides. Moreover,they showed higher similarity in the coding sequences with only a few synonymous SNPs. There were several SNPs and InDels in the second intron,which make a valuable contribution to the genetic diversity of the Psy1 gene,particularly a 163 bp insertion from Psy1-S1h and a 177 bp deletion from Psy1-S1i,which were mainly attributed to the length difference,compared with other Psy1-B1/ Psy1-S1 alleles. |
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