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作者	单位
朱娉，冯波，徐智斌，樊小莉，王涛	（1.中国科学院成都生物研究所，四川成都 610041； 2.中国科学院大学，北京100049）

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中文摘要:

NAM基因是NAC基因家族的成员之一，该基因能加速植株叶片衰老，增加籽粒蛋白质积累，并促进锌、铁等微量元素吸收。为选育高蛋白和高微量元素含量的小麦品种，用NAM共显性标记Xuhw89对858份小麦材料进行了筛选，并进一步扩增全长序列。结果表明，有3个栽培二粒小麦含有功能性NAM基因，其中，F37与F40来源的NAM基因分别与已公布的 TaNAC2基因和 TtNAM-A1基因（GenBank登录号分别为HQ872050和DQ869672）相同；F34来源的NAM基因（命名为TdNAM，GenBank登录号为KU529317）与已公布的 TtNAM-B2基因（GenBank登录号为DQ869676）相似度最高，共有5处核苷酸差异，1处发生在内含子，另外4处发生在外显子区，导致了3个氨基酸残基的变化，预测分析发现这种差异不会对蛋白功能产生影响。因此，本研究筛选获得的NAM基因新等位变异可为进一步选育高蛋白、高微量元素含量的小麦品种提供材料和基因资源。

英文摘要:

NAM gene is a member of the NAC family.It can accelerate leaf senescence,and increase the protein remobilization to grains and promote absorption of zinc,iron and other trace elements.To select the excellent quality wheat with the high protein content and high trace elements,858 wheat lines were screened by co-dominant marker Xuhw89 and the full-length of NAM gene were amplified.Our results showed there were three Triticum dicoccum varieties possess the functional NAM.The nucleotides sequences analysis showed that NAM of F37 and F40 were respectively consistent with the TaNAC2(GenBank No.HQ872050) and TtNAM-A1(GenBank No.DQ869672).The NAM of F34 had the closest homology with the TtNAM-B2(GenBank No.DQ869676).Compared to TtNAM-B2,five variation sites were found in F34,including one difference in intron and four in exons,resulting in three amino acid variations.The prediction analysis showed that the variation had no effect on the protein function.This new allelic variation of NAM gene was named as TdNAM(GenBank No.KU529317）.In this study,the new allele of NAM could provide the materials and gene resources for breeding wheat varieties with high protein and trace elements content.

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