李 蓉,洪 赫,姜文筱,王艳斌,张红娟,赵 芳,刘杰辉,张 泽, 梁 艳,王春静,谢咸升.贝莱斯芽胞杆菌LQ-3防治小麦纹枯病的效果及根际定殖能力[J].麦类作物学报,2024,(7):947 |
贝莱斯芽胞杆菌LQ-3防治小麦纹枯病的效果及根际定殖能力 |
Biocontrol Effect of Bacillus velezensis LQ-3 against Wheat Sharp Eyespot and Its Colonization Dynamics in the Rhizosphere of Wheat |
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DOI:10.7606/j.issn.1009-1041.2024.07.015 |
中文关键词: 贝莱斯芽胞杆菌LQ-3 小麦纹枯病 定殖 防效 |
英文关键词:Bacillus velezensis LQ-3 Wheat sharp eyespot Colonization Biocontrol effect |
基金项目:山西省基础研究计划(自由探索类)青年项目(20210302124679);山西省现代农业产业技术体系建设专项资金(2023CYJSTX02-18);农业基础性长期性科技工作农用微生物观测监测任务(NAES-AM-009);农业基础性长期性科技工作植物保护监测(NAES-PP-017) |
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中文摘要: |
小麦纹枯病(wheat sharp eyespot)主要是由禾谷丝核菌(Rhizoctonia cerealis)侵染引起的一种土传真菌病害,严重威胁小麦生产安全。目前,利用生防细菌防治小麦纹枯病已成为研究热点,其中,研究最多的一类生防细菌为芽胞杆菌。为明确贝莱斯芽胞杆菌(Bacillus velezensis)LQ-3防治小麦纹枯病的效果及其在小麦根部定殖能力,本研究利用LQ-3发酵液灌根处理小麦苗,调查统计LQ-3对小麦纹枯病的防效。同时利用绿色荧光蛋白标记菌株LQ-3(pGFP78)灌根处理小麦根部,并结合抗生素平板回收检测、激光共聚焦显微镜观察方法,探明LQ-3在小麦根部的定殖动态。结果表明,LQ-3防治小麦纹枯病的效果为57.49%,与化学药剂27%苯醚·咯·噻虫悬浮种衣剂(酷拉斯)的防效(59.95%)相当。LQ-3(pGFP78)的生长趋势与野生型LQ-3相比并没有显著性差异,且仍具有游动性和生物膜形成能力。 LQ-3(pGFP78)发酵液初始浓度为2.3×108 cfu·mL-1,灌根处理5 d后,其在小麦根部的定殖量已达到9.63×106cfu·g-1,25 d后,定殖量仍有1.33×106cfu·g-1,表明LQ-3在小麦根部具有较好的定殖能力。 |
英文摘要: |
Wheat sharp eyespot, primarily caused by the soil-borne fungal pathogen Rhizoctonia cerealis, poses a severe threat to wheat production security. Currently, the utilization of biocontrol bacteria for the prevention and control of wheat sharp eyespot has emerged as a research hotspot, with Bacillus being the most extensively studied biocontrol bacteria. To determine the biocontrol effect of Bacillus velezensis LQ-3 on wheat sharp eyespot and its colonization ability in wheat root, this study utilized LQ-3 fermentation to irrigate wheat root, aiming to investigate the biocontrol effect of LQ-3 against wheat eye spot. The colonization dynamics of LQ-3 in wheat root were elucidated by using green fluorescent protein labeled strain LQ-3 (pGFP78) to irrigate wheat root, combined with antibiotic plate recovery detection and laser confocal microscopy observation. The results showed that the biocontrol effect of LQ-3 on wheat sharp eyespot is 57.49%, which is equivalent to the biocontrol effect of the Kuras at 59.95%. Moreover, the growth trend of LQ-3 (pGFP78) exhibited no significant disparity when compared to the wild-type LQ-3, while still retaining its motility and biofilm-forming abilities. The initial concentration of LQ-3 (pGFP78) fermentation broth was 2.3×108 cfu·mL-1, and after 5 d of root irrigation treatment, the colonization of LQ-3 (pGFP78) in wheat root reached 9.63×106 cfu·g-1; even after 25 d, the colonization quantity still remained at 1.33×106 cfu·g-1, indicating that LQ-3 has proficient colonization capability in wheat root. |
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