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作者	单位	地址
刘榛	西北农林科技大学生命科学学院	陕西杨凌邰城路3号西北农林科技大学南校区科研楼1111
怀宝玉
康振生
刘杰^*	西北农林科技大学生命科学学院	陕西杨凌西农路22号西北农林科技大学生命科学学院E305室

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中文摘要:

为了探究小麦TaSTP3基因参与的糖转运途径和其在小麦与条锈菌互作中的作用,以TaSTP3的RNA沉默转基因小麦T2代植株和Fielder小麦植株为材料进行转录组(RNA-seq)测序,筛选差异表达基因并进行GO富集和KEGG分析,推测TaSTP3基因可能参与的调控通路和涉及的基因数量。结果表明,有7660个差异表达基因,其中3194个上调表达,4466个下调表达。GO功能富集发现差异表达基因主要集中在糖类、碳水化合物及纤维素的代谢与合成；KEGG富集分析表明TaSTP3基因沉默后糖类、氨基酸和脂质的代谢与合成中的基因的表达量发生显著变化,并且还有较多基因富集到植物激素信号转导和苯丙烷类物质生物合成通路。综上所述,推测TaSTP3通过运输糖类物质来改变糖类在条锈菌与小麦互作中的分布情况,以此传递分子信号来影响其他营养物质的代谢、植物激素转导和光合作用,因此可以从上述通路中寻找与TaSTP3互作的靶标基因,并对其参与的调控通路展开验证。

英文摘要:

In order to investigate the sugar transport pathway involved in wheat TaSTP3 gene and its role in wheat-stripe rust interactions, TaSTP3-RNAi transgenic wheat T2 generation plants and Fielder wheat plants were used for RNA-seq, to screen for differentially expressed genes(DEGs) and to use GO and KEGG analyses to speculate on the possible involvement of the TaSTP3 gene in the regulatory pathway and the number of genes involved. The results showed that there were 7660 DEGs, of which 3194 were up-regulated and 4466 were down-regulated. GO functional enrichment revealed that the DEGs were mainly concentrated in the metabolism and synthesis of sugars, carbohydrates, and cellulose; KEGG enrichment analysis showed that the expression of genes in the metabolism and synthesis of sugars, amino acids and lipids changed significantly after TaSTP3 gene silencing, and more genes were enriched in the phytohormone signaling and phenylpropanoid biosynthesis pathways. In summary, it is hypothesized that TaSTP3 alters the distribution of sugars in stripe rust-wheat intercropping by transporting sugars, and in this way transmits molecular signals to affect the metabolism of other nutrients, phytohormone transduction, and photosynthesis, so that target genes interacting with TaSTP3 can be searched for from the above pathways, and their involvement in the regulatory pathways can be verified.

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