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| 硫酸铜胁迫对小麦幼苗生长的影响 |
| Effect of CuSO4 stress on the growth of wheat seedling |
| 投稿时间：2023-12-24 修订日期：2024-04-08 |
| DOI： |
| 中文关键词: 小麦，硫酸铜，幼苗，抗氧化酶活性，有丝分裂 |
| 英文关键词:Wheat, CuSO4, Seedlings, Antioxidant activity, Mitosis |
| 基金项目:国家自然科学(31500321)第一作者1084593043@qq. com(许世初)通讯作者ywmo@163. com(莫亿伟) |
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| 中文摘要: |
| 为了探讨Cu2+胁迫对小麦萌发和生长的影响,本文采用水培法,以1/100 MS培养基作为对照,并在此基础上加入不同剂量的CuSO4母配制30、60和90 mg/L CuSO4溶液用于浸种催芽和培育幼苗,测定不同处理时间点幼苗相关生理指标和生长参数。结果发现,随着CuSO4处理浓度增加,种子淀粉酶活性显著降低,淀粉粒分解速率减弱；种子内可溶性总糖、可溶性蛋白和氨基酸含量均显著低于对照；虽对照组种子SOD和CAT活性显著高于各处理组,但其POD活性则显著低于各处理组。然而,与对照组相比,根系SOD、CAT和POD活性随着CuSO4浓度增加显著增加,但呼吸脱氢酶活性、超氧阴离子(O2.-)含量和H2O2荧光强度显著下降。此外,Cu2+主要积累于根系,表现为根尖有丝分裂明显受阻,导致株高、根长和总根数减少,植株生物量下降。综上所述,CuSO4对小麦幼苗的毒害表现出依赖浓度的剂量效应,且Cu2+可抑制种子萌发阶段的淀粉酶活性使淀粉分解受阻,造成氧化伤害,通过抑制根尖有丝分裂,最终使小麦生长受阻,表现出生理毒性和遗传毒性。 |
| 英文摘要: |
| The effects of Cu2+ stress on the germination and growth of wheat (Triticum aestivuml) seedlings was studied in the present study by using hydroponic method. In which, 1/100 MS medium as the control, and different doses of CuSO4was added in the medium to prepare 30, 60, and 90 mg/L CuSO4 solutions for seed soaking, germination, and seedling cultivation. The relevant physiological indicators and seedling growth parameters at different treat stage were measured. The results showed that CuSO4 stress decreased the activity of seed amylase significantly accompanied by the rate of starch particle decomposition weakening; reduced the contents of total soluble sugar, soluble protein and amino acid, decreased the activities of SOD and CAT but increased POD activity in seeds compared with the control. However, stress environment increased the activities of SOD, CAT and POD but decreased the activity of respiratory dehydrogenase, the content of superoxide anion (O2.-) and the fluorescence intensity of H2O2 significantly in roots. In addition, Cu2+ was accumulatedmainly in roots, which caused the obvious obstruction of root tip mitosis, and resulted in the decrease of plant height, root length and total root number, as well as the plant biomass. In conclusion, the toxicity of CuSO4 to wheat seedlings showed a concentration-dependent dose effect. Cu2+exhibited physiological toxicity and genetic toxicity by inhibiting the amylase activity at the stage of seed germination to hinder the decomposition of starch, inducing by inhibiting root tip mitosis, causing oxidative damage, and ultimately hindering the growth of wheat. |
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