欢迎访问《麦类作物学报》编辑部网站

作者	单位
崔思宇，张志芬，付晓峰，刘俊青，杨海顺	（内蒙古自治区农牧业科学院特色作物研究所，内蒙古呼和浩特 010032）

摘要点击次数: 145

全文下载次数: 190

中文摘要:

植物激素信号转导通路是响应胁迫的重要通路，该通路中基因的表达调控作物的抗旱性。为挖掘燕麦植物激素信号转导通路中响应干旱胁迫的关键基因，本研究对燕麦幼苗进行不同干旱胁迫处理，取叶片进行转录组测序，分析不同处理下基因的表达。结果表明，与正常水分条件相比，轻度干旱胁迫（PEG 10%）诱导624个基因表达发生显著变化（DEGs），重度干旱胁迫（PEG 20%）诱导13 063个。GO富集分析显示，重度干旱胁迫下，DEGs主要富集在对胁迫反应的调节；KEGG分析显示，轻度干旱胁迫下，植物激素信号转导通路中1个ARF和2个CRE1基因表达显著下调，表达量较高，可能为燕麦在该通路中响应轻度干旱胁迫的基因；而重度干旱胁迫下，植物激素信号转导通路富集169个DEGs，其中生长素和脱落酸信号转导过程DEGs较多，分别占植物激素信号转导通路总DEGs的20.7%和15.9%；在8条激素信号转导过程中筛选出12个表达量较高的基因，可能为燕麦在该通路中响应重度干旱胁迫的关键基因。本研究将为今后燕麦抗旱基因的克隆和验证提供依据。

英文摘要:

Plant hormone signal transduction pathway is an important pathway in response to stress, and gene expression in this pathway regulates crop drought resistance. In order to explore the key genes of oat in response to drought stress in plant hormone signal transduction pathway, we conducted different drought stress treatments on oat seedlings, took leaves for transcriptome sequencing, and analyzed the gene expression under different treatments. The results showed that compared with normal water conditions, 624 differentially expressed genes (DEGs) were induced under mild drought stress (PEG 10%) and 13 063 DEGs were induced under severe drought stress (PEG 20%). The enrichment analysis of GO showed that under severe drought stress, DEGs were mainly enriched in the regulation of stress response; KEGG analysis showed that under mild drought stress, the expression of one ARF gene and two CRE1 genes in plant signal transduction pathway was significantly down regulated, and the expression level was high, which may be the genes of oat in response to mild drought stress in this pathway. Under severe drought stress, 169 DEGs were enriched. Among them, more DEGs were involved in auxin and abscisic acid signal transduction, accounting for 20.7% and 15.9% of the total DEGs in plant hormone signal transduction pathway. Twelve genes with high expression level were screened from eight hormone signal transduction processes, which may be the key genes for oats to respond to drought stress. This study will provide a basis for the cloning and verification of drought resistance genes in oats in the future.

查看全文查看/发表评论下载PDF阅读器

关闭