| 宋 晓，黄绍敏，张珂珂，王沙沙，李向东，杨 程，杨天军.小麦硝酸盐转运蛋白TaNRT1.1 1A转录活性检测及互作蛋白筛选[J].麦类作物学报,2023,(10):1227 |
| 小麦硝酸盐转运蛋白TaNRT1.1 1A转录活性检测及互作蛋白筛选 |
| Detection of Transcription Activity and Screening of Interaction Proteins of Nitrate Transporter 1/Peptide Transporter TaNRT1.1-1A in Wheat |
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| DOI： |
| 中文关键词: 小麦 TaNRT1.1-1A 转录激活 酵母双杂交 互作蛋白 |
| 英文关键词:Triticum aestivum L. TaNRT1.1-1A Transcriptional activation Yeast two-hybrid system Interacting protein |
| 基金项目:河南省科技研发计划联合基金(222103810015)；河南省农业科学院自主创新项目(2022ZC26) |
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| 中文摘要: |
| 为了进一步解析小麦硝酸盐转运蛋白TaNRT1.1-1A的调控机制，本研究对其进行了自激活性检测。利用已构建好的小麦酵母cDNA文库，以TaNRT1.1-1A为诱饵，通过酵母双杂交技术筛选其互作蛋白，回转试验进一步验证其互作关系。结果表明，TaNRT1.1-1A无自激活活性，酵母双杂技术筛选小麦cDNA文库，共获得2个候选互作蛋白，候选蛋白Sdr I-like主要参与植物病害有关的应答响应。Sdr I-like的回转验证结果表明，该蛋白可能与TaNRT1.1-1A存在互作关系。该结果可为进一步研究TaNRT1.1-1A调控小麦硝酸盐吸收有关的分子机制奠定基础。 |
| 英文摘要: |
| To further explore regulatory mechanism of Nitrate Transporter 1/Peptide Transporter TaNRT1.1-1A in wheat, the self-activation of TaNRT1.1-1A was detected in this study. The TaNRT1.1-1A was used as bait to screen wheat yeast cDNA library by the yeast two-hybrid system. The protein interaction between key candidate proteins and TaNRT1.1-1A gene was validated. The results showed that TaNRT1.1-1A had no self-activation activity. Two candidate interacting proteins of TaNRT1.1-1A were obtained, of which the candidate protein Sdr I-like was mainly involved in plant disease. The validation assays of candidate protein Sdr I-like indicated that the protein was probably interacted with TaNRT1.1-1A. This study lays a foundation for further study on the molecular mechanism of TaNRT1.1-1A in regulating the nitrate uptake process in wheat. |
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