| The chemical hybridization agent SQ-1 induces wheat pollen abortion, which is a complex physiological and metabolic process, and TaMYB5-like has been found to be involved in this process. To understand the role of TaMYB5-like gene in the process of SQ-1 induced physiological pollen abortion in wheat, the TaMYB5-like gene was cloned.The SQ-1 induced physiological male sterile line PHYMS-1376 and the control fertile line CK-1376 treated with equal amounts of water were used as experimental materials, including tetrad, early mononuclear, late mononuclear, binuclear, and trinuclear anthers. The sequence structure, bioinformatics, and expression patterns of the TaMYB5-like gene were analyzed and subcellular localization and transcriptional self-activation effect were also performed. The results showed that the TaMYB5-like gene sequence was 5 207 bp long, and its cDNA was 1 133 bp long. The CDS sequence was 819 bp long, encoding 272 amino acids, containing two SANT domains, with a molecular weight of 29.36 kDa, but without signal peptide, or transmembrane structure.Subcellular localization showed TaMYB5-like is located in the nucleus. The promoter of TaMYB5-like gene involves cis acting elements such as light response, stress response and methyl jasmonic acid response. The self activation effect found that the self activation region of TaMYB5-like protein is located at the N-terminus, and appropriate concentrations of AbA and 3-AT can inhibit the self activation activity of BD-TaMYB5-like and BD-TaMYB5-like N-SANT fusion proteins. As the amino acid sequence of the protein was truncated, the self activation activity decreased. Compared with CK-1376, there was no significant difference in TaMYB5-like expression levels between the tetrad and early mononuclear anthers of PHYMS-1376 plants. TaMYB5-like expression levels significantly increased during the late mononuclear, binuclear, and trinuclear stages of anther development. The expression levels of TaMYB5-like in the anthers of CK-1376 and PHYMS-1376 were the highest during the trinuclear stage. |
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