| In order to clarify the key factors for the stem formation of the lodging resistant wheat cultivar Qianmai 1175, growth characteristics, stem characteristics, conttents of cellulose and lignin and other traits during the anthesis and maturity stages were comprehensively investigated, with Qianmai 18(easy lodging) as the control. Transcriptome sequencing was performed to reveal the mechanism of stem formation, and GO functional annotation and KEGG enrichment analysis were performed. The results showed that the plant height, gravity center height and ear length of Qianmai 1175 were significantly lower than those of Qianmai 18 at flowering stage, while there was no significant differences in the ear weight, stem length and weight, basal 1-2 internode length and weight. At maturity stage, the ear length, stem gravity center height, length and weight of Qianmai 1175 were significantly lower than those of Qianmai 18, but there was no significant differences in ear weight, basal 1-2 internode length and weight. The stem lodging resistance, cellulose and lignin of Qianmai 1175 were significantly higher than those of Qianmai 18. Transcriptome sequencing showed that clean reads of each sample for transcriptome analysis reached above 13.85 Gb, and the percentage of Q30 base was above 93.8%. GO function classification and KEGG pathway enrichment analysis were performed on differentially expressed genes(DEGs). DEGs were mainly distributed in catalytic activity, binding, metabolic process, cell part, membrane part, cell process, organelle membrane, stimulation response, biological regulation, transport activity and transcriptional regulation activity. KEGG pathway contained phenylpropane biosynthesis, DNA replication, photosynthesis antenna protein and plant hormone signal transduction. |
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