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作者	单位
车卓，张沛沛，陈涛，刘媛，马靖福，田甜，王鹏，杨德龙	(1.甘肃农业大学生命科学技术学院,甘肃兰州 730070； 2.甘肃省种子总站,甘肃兰州 730070；3.干旱生境作物学国家重点实验室,甘肃兰州 730070)

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中文摘要:

葡萄糖-6-磷酸脱氢酶(G6PDH)是磷酸戊糖途径中的关键限速酶，在植物生长发育及非生物胁迫响应中发挥重要作用。本研究利用生物信息学对小麦 G6PDH基因家族成员进行鉴定，并对该家族基因编码蛋白的理化性质、进化关系、亚细胞定位、基因复制事件以及在不同组织和不同胁迫下的表达模式进行分析。结果表明，在小麦基因组中共鉴定到14个 G6PDH基因家族成员，分布在小麦2A、2B、2D、4A、4B、4D、6A、6B和6D染色体上，其中5个基因编码的蛋白为胞质型，9个为质体型。亚细胞定位预测表明，胞质型G6PDH蛋白主要定位于细胞质上，而质体型G6PDH蛋白主要定位于叶绿体上。根据系统进化和保守结构域特征，可将14个小麦G6PDH蛋白分为Cy、P0、P1和P2四个亚组。共线性分析表明，小麦 G6PDH基因家族成员存在17对片段重复基因。RNA-seq分析结果表明，小麦 G6PDH基因家族成员在不同组织中存在明显的表达差异，其中 TaG6PDH1-2A/2B/2D基因在根中的表达量最高； TaG6PDH2-2A和 TaG6PDH2-2B基因在干旱胁迫后1 h以及热胁迫后6 h均上调表达。进一步利用qRT-PCR检测6个小麦 G6PDH基因在干旱和盐胁迫下的表达模式，发现分别有5个基因在小麦根中均上调表达，推测小麦 G6PDH基因在非生物胁迫响应过程中发挥着重要功能。

英文摘要:

Glucose-6-phosphate dehydrogenase(G6PDH) is recognized as a key enzyme in plant pentose phosphate pathway,which is involved in regulating plant growth and development and abiotic stress response. In this study,wheat G6PDH gene family members were identified using bioinformatics methods,and their physical and chemical properties,evolutionary relationship,subcellular localization,gene replication events,as well as the expression patterns of different tissues under drought and salt treatment were analyzed. The results showed that a total of 14 G6PDH family members in wheat genome were identified,unevenly distributing on 2A,2B,2D,4A,4B,4D,6A,6B and 6D chromosomes. Among them,there were five cytosolic NADP⁺-dependent isoforms,and nine plastidic NADP⁺-dependent isoforms. Subcellular localization prediction showed that cytosolic isoforms were localized in the cytoplasm,and plastidic isoforms were mainly localized in the chloroplast. According to phylogenetic analysis,these 14 TaG6PDH were divided into four subclasses,containing Cy,P0,P1,and P2. Collinearity analysis indicated that there are 17 pairs of segmental duplication genes in wheat G6PDH gene family. RNA-seq analysis showed that G6PDH gene family members in wheat exhibited preferential expression in specific tissues. Among them, TaG6PDH1-2A/2B/2D were highest expressed in root tissues. The expression of TaG6PDH2-2A and TaG6PDH2-2B genes was up-regulated at 1 h after drought stress and 6 h after heat stress. Furthermore,qRT-PCR was used to detect the expression patterns of six wheat G6PDH genes under drought and salt stresses. It was found that five wheat G6PDH genes were up-regulated in wheat roots,which suggested that the wheat G6PDH gene played an important role in abiotic stress response.

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