| Thinopyrum ponticum is an important gene source of wheat, which has many excellent traits, such as cold resistance, drought resistance, salt tolerance, thick stems and long panicles. In this study, CH18067, derived from a cross between common wheat 7182 and Th. ponticum, was identified by morphology, cytogenetic, in situ hybridization, molecular markers and disease resistance. The results of cytogenetic observation showed that CH18067 has 42 chromosomes, with the configuration of 2n=21Ⅱ at metaphase I of meiosis, which can be segregated equally at anaphase I of meiosis. Fluorescence in situ hybridization(FISH) with oligo nucleotide probes Oligo-pTa535(red) and Oligo-pSc119.2(green) showed that CH18067 lacked the 2D and 4D chromosomes of wheat and contained two pairs of chromosomes with special banding patterns. Two pairs of special banding chromosomes were identified by FISH-GISH, mc-GISH, chromosome karyotype analysis, and GenoBaitsWheatplus EE liquid array detection of CH18067. It was found that the chromosomes showing Oligo-pTa535 probe red banding at the end of the long arm and the short arm were the 2J chromosomes of Th. ponticum, and the chromosomes showing Oligo-pTa535 probe red banding at the centromere position and the end of the long arm were the 4JS chromosomes of Th. ponticum. The results indicate CH18067 was a wheat-Th. ponticum 2J(2D)+4JS(4D) double substitution line. Seven specific markers were screened, which could be used to track 2J and 4JS chromosomes of Th. ponticum in wheat genetic background. Morphological investigation and identification of stripe rust resistance showed that CH18067 had the characteristics of dwarf and long grain, and it was highly resistant to stripe rust at adult stage. Therefore, CH18067 can be used as a candidate germplasm for wheat stripe rust resistance breeding and genetic research. |
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