| In order to clarify the pathogen of naked barley Fusarium root rot in Hezuo city,Lintan County,Zhuoni County,Gannan Tibetan Autonomous Prefecture,Gansu Province,the occurrence of naked barley root rot was investigated at seedling stage and adult stage and samples were collected. The pathogen was separated by the conventional tissue separation method.The classification status of pathogens was determined by combining morphological characteristics with molecular biological identification,and the pathogenicity was determined by beaker-water agar and pot-culture method. The results showed that the naked barley Fusarium root rot was widely distributed in Gannan Tibetan Autonomous Prefecture of Gansu Province,and the occurrencerate was between 5% and 20%.The disease plant was weak and yellow,with constricted and rotted stem base,white ear,hollow grain and brown or dark red stem.A total of 88 strains of naked barley Fusarium root rot were identified,including 50 strains at seedling stage and 38 strains at adult stage. They were identified as Fusarium avenaceum,F.equiseti,F.tricinctum,F.flocciferum,F.acuminatum and F.langsethiae. The difference of pathogenicity between pot-culture with beaker-water agar method was significant,but both indicated that F.avenaceum had strong comprehensive pathogenicity. Combined with its isolation rate,F.avenaceum was confirmed as the dominant pathogen. |
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