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作者	单位
刘佳奇,李丽,杨红红,孙敏,冯汉青	（1. 西北师范大学生命科学学院，甘肃兰州 730070；2. 西北师范大学新农村发展研究院，甘肃兰州 730070）

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中文摘要:

盐碱地会严重影响小麦种子的萌发和幼苗的生长。为了探究褪黑素对盐胁迫下小麦种子萌发和幼苗生长的影响，研究了NaCl（100 mmol·L^-1）胁迫下不同浓度的褪黑素（5、50、100、150、250 μmol·L^-1）对小麦种子萌发、幼苗生长、光化学活性、叶绿素含量以及根细胞活力的影响。结果发现，100 μmol·L^-1褪黑素能够显著缓解NaCl胁迫对小麦种子萌发的抑制作用；100~150 μmol·L^-1褪黑素可明显改进盐胁迫下小麦幼苗的生长状况，显著缓解盐胁迫对PS （光系统）Ⅱ光化学活性的抑制和小麦幼根细胞死亡的发生；150 μmol·L^-1褪黑素显著促进了小麦叶片叶绿素合成。综合分析表明，施用外源褪黑素可有效缓解盐胁迫对小麦造成的萌发率、光化学活性、叶绿素含量下降、生长迟缓、根细胞死亡等不利影响，以100~150 μmol·L^-1褪黑素的缓解效果最为明显。

英文摘要:

Saline-alkali soil seriously affects wheat seed germination and seedling growth. In order to explore the effect of melatonin on wheat seed germination and seedling growth under salt stress,we investigated the alleviating effects of exogenous melatonin at different concentrations(5, 50, 100, 150 and 250 μmol·L^-1) on wheat seed germination, seedling growth,photochemical activity of PS Ⅱ,the content of chlorophyl Ⅱ and root cell death under NaCl stress.The results showed that melatonin at 100 μmol·L^-1significantly alleviated the decrease of seed germination caused by NaCl stress;100-150 μmol·L^-1 melatonin significantly improved the growth of wheat seedlings under salt stress; 100-250 μmol·L^-1melatonin significantly alleviated the inhibition of NaCl stress on the photochemical activity of PS Ⅱ and reduced the increase in the level of cell death under NaCl stress. Further study showed that 150 μmol·L^-1 melatonin significantly promoted synthesis of chlorophyl Ⅱ in wheat leaves.In conclusion, exogenous melatonin effectively alleviated the negative effects of salt stress on wheat seed germination, photochemical activity and chlorophyl Ⅱ content, seedling growth retardation, and root cell death. The alleviative effect of 100-150 μmol·L^-1melatonin is the most effective.

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