| Grain weight stability is an important evaluation index of wheat yield stability. In order to find out the QTLs that affect the stability of grain weight in wheat under reduced source stress such as insect damage,the phenotypic and QTL analysis of grain length,grain width and 1 000 grain weight were carried out in the RILs population derived from the cross between Xiaoyan 81 and Xinong 1376 under four environments of flag leaf removal as well as early sowing and late sowing. At the same time,the phenotypic ratios of source reduction treatment to control treatment was calculated,and the stability was estimated by the ratio to find the QTLs of ratios of phenotype,and the high-density genetic linkage map was constructed by using 50K SNP assay. The result showed that compared with the control treatment,the grain length,grain width and 1 000-grain weight of RIL population were significantly decreased under source reduction treatment. And there were 18 QTLs distributed on 2A,3A,3B,3D,4B,4D and 5D chromosomes,8 QTLs controlling grain length,grain width and 1 000 grain weight were expressed in source reduction treatment and control treatment,in which Qgl.nwafu-5D.3 and Qkgw.nwafu-5D may be the new locus,and Qgl.nwafu-5D.3 andQkgw.nwafu-5D were stable QTLs with the average phenotypic vatiation of 8.73% and 8.19%,which were detected under control treatment at early sowing and source reduction treatment at late sowing,and Qgl.nwafu-5D.3 was also detected under source reduction treatment at early sowing. Most QTLs controlling phenotypic ratios coincide with those controlling grain length,grain width and 1 000 grain weight,it is speculated that QTLs of maintaining the stability of grain weight after flag leaf removal are closely related to QTLs of grain length,grain width and 1 000 grain weight or had “one cause,multiple effects”. |
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