| Drought is one of the main abiotic stresses that affect the yield of barley.Exploring drought response genes of barley is beneficial to variety improvement. In this study,we analyzed the population evolution of 167 Central Asian barley accessions under three irrigation regimes,namely normal irrigation(irrigating at seedling,elongation,booting,heading and filling stages),irrigation twice(irrigating at seedling and booting stages) and irrigation once(irrigating at seedling stage). Plant height,ear length,spikelet number and spike number per plant were evaluated for genome-wide association study(GWAS). We found that there were no obvious geographical subgroups. A total of eight QTL loci were found to be associated with the four agronomic traits. Further functional analysis identified 17 candidate genes,and there were 7,4,4 and 2 candidate genes regulating plant height,ear length,spikelet number and spike number per plant of barely,respectively. At the same time,GWAS was performed according to the drought index(DI) phenotype,and seven QTLs were identified on 1H,4H,5H and 7H chromosomes,underlying 96 promising candidate genes. This study provides basic data for further molecular breeding and cloning functional genes for drought resistance in barley. |
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