| Jasmonic acid(JA) plays an important role in plant response to low temperature stress.Our previous study found that the strong cold resistance of Dongnongdongmai 1(Dn1) was related to JA signal transduction,and exogenous MeJA treatment improved the cold resistance of Dn1. In order to explore whether the improvement of cold resistance of Dn1 by JA is related to the expression of endogenous JA synthesis genes,the expression levels of JA synthesis genes TaLOX1 , TaLOX2 , TaLOX3 ,TaAOS,TaAOC, TaOPR2 , TaLOX2A , TaLOX2B and TaLOX2D under different temperature stress were quantitatively analyzed by real-time PCR. The functions of TaLOX2A,TaLOX2B and TaLOX2D proteins were predicted by bioinformatics analysis. The results showed that low temperature stress increased the relative expression of TaLOX1, TaLOX3,TaAOC and TaOPR2 in Dn1 tillering nodes,with the highest expression at -10 ℃; Exogenous MeJA treatment significantly increased the expression of TaLOX1, TaLOX2, TaLOX3,TaAOS and TaAOC at 0 ℃; TaLOX2A, TaLOX2B and TaLOX2D genes were located on chromosome 5 of wheat,and their coded proteins were located in the cytoplasm,belonging to random coil proteins.The structure,conserved domain and function of the proteins are same or similar. This study provides a theoretical basis for further revealing the role of JA synthetic genes in winter wheat response to cold stress. |
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