| 姚琦馥，魏佳泰，李水琴，刘 航，马 建.大麦旗叶长和宽的QTL分析[J].麦类作物学报,2021,(11):1365 |
| 大麦旗叶长和宽的QTL分析 |
| QTL Mapping for Barley Flag Leaf Length and Width |
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| DOI：10.7606/j.issn.1009-1041.2021.11.06 |
| 中文关键词: 大麦 旗叶长 旗叶宽 QTL 聚合效应 |
| 英文关键词:Barley Flag leaf length Flag leaf width QTL Pyramiding effect |
| 基金项目:四川省应用基础研究计划项目（2020YJ0140，2021YJ0503）；四川省国际合作交流项目（2021YFH0083）；贵州省梵净山地区生物多样性保护与利用重点实验室建设项目(黔科合平台人才［2020］2003)；贵州省科技计划基础研究项目（黔科合基础-ZK［2021］一般131） |
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| 中文摘要: |
| 为解析调控大麦旗叶大小的遗传机制，以1个大麦重组自交系(RIL)群体(由J36528和BMJ89为亲本构建，包含125个F10代)为材料，利用447个DArT和8个SSR标记绘制遗传连锁图谱，结合4年2点共6个生态环境下测得的旗叶长和宽表型数据，鉴定旗叶长和宽相关的QTL。结果表明，所构建的遗传图谱包含7个连锁群，总长1 034.96 cM，平均每条染色体147.85 cM，标记密度为2.27 cM。共检测到7个旗叶长QTL和5个旗叶宽QTL，分布在2H、4H、5H、6H和7H染色体上。其中，有2个旗叶长主效位点 QFll.sicau-JB-5H.2和 QFll.sicau-JB-6H.1，能够在多环境下稳定表达，表型变异解释率范围分别为12.02%~ 19.95%和16.69%~16.99%；二者累加对旗叶长具有增加效应，聚合这2个位点对大麦旗叶长的调控和产量提升具有潜在价值。另外，有1个旗叶宽主效位点 QFlw.sicau-JB-4H在多环境下能够稳定表达，表型变异解释率为15.03%~23.99%。将主效QTL锚定到大麦参考基因组后比对发现， QFll.sicau-JB-6H.1可能为新位点，而 QFll.sicau-JB-5H.2可能与小麦第五部分同源群检测到的旗叶长位点具有同源性。本研究鉴定获得的与旗叶长和宽相关的稳定表达的主效QTL对大麦不同旗叶形态基因的精细定位和分子辅助育种提供了依据。 |
| 英文摘要: |
| To uncover genetic mechanism of barely flag leaf-related traits,a genetic linkage map containing 447 DArT and 8 SSR markers was constructed in the F10 RIL population consisting of 125 lines constructed by J36528 and BMJ89,and QTLs related to flag leaf length and width were identified by combining data from six environmentsat two locations in four years. The constructed genetic map consists of seven linkage groups with a total length of 1 034.96 cM,an average of 147.85 cM per chromosome,and a marker density of 2.27 cM/marker. A total of seven QTLs of flag leaf length and five flag leaf width were detected on the 2H,4H,5H,6H and 7H chromosomes. QFll.sicau-JB-5H.2 and QFll.sicau-JB-6H.1 for flag leaf length,are major and stably expressed in multiple environments,explaining 12.02%-19.95% and 16.69%-16.99% of the phenotypic variance,respectively. Genetic analysis showed the combination of QFll.sicau-JB-5H.2 and QFll.sicau-JB-6H.1 had the largest effect on flag leaf length,suggesting the potential of pyramiding these two QTL in regulating flag leaf length and thus possibly improving barley yield. Additionally,a major QTL QFlw.sicau-JB-4H for flag leaf width was stably detected in multiple environments with 15.03%-23.99%of explained phenotypic variance. Mapping major QTL on barley reference genome suggested that QFll.sicau-JB-6H.1 was most likely a novel QTL,while QFll.sicau-JB-5H.2 was likely orthologous to loci for flag leaf length on the long arm of the fifth homologous groups in wheat. In this study,the identified major and stably expressed QTLs associated with flag leaf length and width provide a basis for fine mapping and molecular assisted breeding in barley. |
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