欢迎访问《麦类作物学报》编辑部网站

作者	单位
魏迪，常平，刘佳熠，杨阳，张璇，陈亮，胡银岗	（西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室，陕西杨凌 712100）

摘要点击次数: 614

全文下载次数: 535

中文摘要:

表皮模式因子EPF/EPFL基因家族编码一类植物中特有的分泌类蛋白，在植物生长发育特别是形态建成过程中发挥着重要作用。为挖掘和利用小麦EPF/EPFL家族基因，对小麦该家族基因进行了系统鉴定，并利用生物信息学技术对其基因结构、蛋白质结构域、系统进化及表达特性进行分析。结果表明，在小麦全基因组水平共鉴定到35个TaEPF/EPFL基因家族成员，含有1~4个外显子，分布在除1A、5B外的19条染色体上，全基因组复制事件是导致该基因家族扩张的主要原因。蛋白结构分析显示，其编码蛋白的C端包含6个相对保守的半胱氨酸残基，N端存在信号肽剪切位点，且亚细胞定位在胞外基质中，属于一类胞外分泌蛋白。系统进化分析发现，TaEPF/EPFL基因在单子叶和双子叶植物分化之前就已形成。表达模式分析发现，大多数TaEPF/EPFL基因在小麦幼嫩组织和穗部表达量较高，部分TaEPF/EPFL基因响应干旱、高温等非生物胁迫。进一步分析 TaEPF1-2B不同单倍型与气孔性状的相关性，发现TaEPF1-2B不同单倍型的气孔密度、气孔长度、气孔宽度和气孔面积均存在极显著差异，净光合速率存在显著差异，其中单倍型Hap A具有较低的气孔密度和较高的光合速率，是优势单倍型。本研究结果为进一步改良小麦的抗旱性和光合效率提供了候选基因。

英文摘要:

The EPF/EPFL family genes encode a class of plant-specific secreted proteins,play an important role in plant growth and development,especially in the process of plant morphogenesis. In order to explore and utilize EPF/EPFL genes family in wheat,In this study,wheat EPF/EPFL genes were systematically identified at genome level. Subsequently,gene structure,conserved domain of proteins,phylogenic relationship,and expression characteristics were analyzed using bioinformatics methods. The result showed that 35 TaEPF/EPFL genes were identified with 1-4 gene exons,and distributed on 19 chromosomes expect 1A and 5B. Whole genome replication events were the main reason for the expansion of this gene family. Protein structure analysis revealed that TaEPF/EPFL proteins belong to the extracellular secretory proteins with C-terminus containg 6 relatively conserved cysteine residues and N-terminus containing a signal peptide cleavage site,which is subcellular localization in the extracellular matrix. Phylogenetic analysis showed that TaEPF/EPFL gene was formed before the differentiation of monocotyledons and dicotyledons. Expression pattern analysis revealed that most TaEPF/EPFL genes had high and specific expression levels in the young tissues and spikes,and some TaEPF/EPFL genes expressed in response to abiotic stresses such as drought stress and heat stress.The correlation between stomatal characteristics and haplotype of TaEPF1-2B was further analyzed,found that two haplotypes of TaEPF1-2B had extremely significant differences in stomatal frequency,stomatal length and width,and stomatal area. And TaEPF1-2B haplotypes had significant differences in net photosynthetic rate. Hap A was the superior haplotype with lower stomatal frequency and higher net photosynthetic rate. These results provide candidate genes for improving drought-resistance and photosynthetic efficiency in wheat.

查看全文查看/发表评论下载PDF阅读器

关闭