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芒是小麦穗部重要的光合器官之一，是小麦长期进化和适应环境的产物，对小麦产量和逆境适应具有重要影响。为了探究芒的发育及芒长的遗传机制，本研究利用小麦无芒品种中国春（Chinese Spring，CS）和有芒品系MK147构建的F₂分离群体与F₅代重组自交系（RIL）群体为材料，对无芒位点进行了标记和定位。遗传分析表明，群体中的无芒性状受半显性单基因控制；采用Wheat660K SNP芯片结合集群分离分析法（bulked segregate analysis，BSA）在F₂群体构建的无芒、有芒混池中检测到一个多态性SNP标记的富集区，初步将QTL定位于4A染色体上。在该QTL区间开发了58个KASP标记，并将定位区间缩小至KASP标记SNP-1537与SNP-4195之间，遗传距离为0.81 cM，对应中国春参考基因组（IWGSC.Ref.V1.0）4A染色体上9.23 Mb （100.56～109.79 Mb）的区间，将该位点命名为 Awn-4A.1。

英文摘要:

Awn is one of the important photosynthetic organs in the ear of wheat. It derived from the long-term evolution and adaptation of wheat, and has an important impact on wheat yield and adaptation to the environments. In order to explore the development of awn and the genetic mechanism of awn length, we used F₂ segregation population and RIL population constructed by Chinese Spring (CS) and MK147 as materials. Genetic analysis showed that the awnless character in population was controlled by semi-dominant single gene. Using Wheat660K SNP and bulk segregate analysis (BSA) method, a polymorphic SNP marker enrichment region was detected in F₂ constructed awnless and awn mixed pool, and the QTL was initially located on 4A chromosome. A total of 58 KASP markers were developed in the candidate region, and the mapping interval was narrowed down between KASP marker SNP-1537 and SNP-4195, with a genetic distance of 0.81 cM on chromosome 4A,correspond to the 9.23 Mb(100.56-109.79 MB) on 4A chromosome of Chinese Spring reference genome(IWGSC.Ref. v1.0), the locus was assigned as Awn-4A.1.

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