To under stand the physiological characteristics of wheat flag leaf at the grain filling stage and the response of wheat yield to irrigation treatments three wheat varieties,Heng 6632(H6632), Shinong 086(S086) and Jimai 22(J22),were used in the experiment. Three treatments,no irrigation in spring(W0), irrigation at jointing(W1, 75 mm), irrigation at jointing and flowering(W2, 150 mm),were set up to analyze the effect of different irrigation amount on the antioxidant and osmotic adjustment ability of flag leaf at grain filling and yield. The results showed that under the treatments of W1 and W2, the superoxide dismutase(SOD) activity, peroxidase(POD) activity and catalase(CAT) activity of different wheat varieties were significantly higher than those of W0, and the content of aldehyde(MDA), proline(Pro) and soluble sugar were significantly lower than those of W0,the yield and grain number per spike were significantly higher than those of W0, and the yield of S086 and J22 under W2 were significantly higher than that of W1. For W1 and W2 in the field treatments, the activity of SOD, POD and CAT of different wheat varieties were significantly higher than that of W0, and the contents of Pro and soluble sugar were significantly lower than those of W0. The yield and effective ear number of W1 and W2 of three wheat varieties were significantly higher than those of W0. The yield of different wheat varieties were not significantly different from that of W2 and W1. This shows that spring irrigation can increase the antioxidant ablity of wheat to varying degress and increase grain yield. |