| 李 桃,汪军成,姚立蓉,李葆春,马小乐,杨 柯,司二静,尚勋武,王化俊,孟亚雄.大麦在网斑病菌侵染后的转录组学分析[J].麦类作物学报,2020,(9):1070 |
| 大麦在网斑病菌侵染后的转录组学分析 |
| Transcriptomic Analysis of Barley(Hordeum vulgare) Infected by Pyrenophora teres |
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| DOI：10.7606/j.issn.1009-1041.2020.09.07 |
| 中文关键词: 大麦 网斑病菌侵染 转录组分析 差异表达基因 |
| 英文关键词:Barley Pyrenophora teres infection Transcriptome analysis Differentially expressed gene |
| 基金项目:甘肃省现代农业产业技术体系特色作物岗位专家资助项目（GARS-TSZ-2）；国家大麦青稞产业技术体系专项（CARS-05-03B-03）；甘肃省重大科技专项（17ZD2NA016）；甘肃农业大学学科建设专项（GAU-XKJS-2018-082，GAU-XKJS-2018-083）；盛彤笙科技创新基金项目（GSAU-STS-1513，GSAU-STS-1735)；国家自然科学基金项目(30771331)；农业产业技术体系建设项目(CARS-05) |
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| 中文摘要: |
| 近年来，由子囊菌亚门真菌网斑病菌(Pyrenophora teres)引起的大麦网斑病在我国大麦（Hordeum vulgare L.)主产区大面积发生并流行，导致大麦产量和品质下降。为探索大麦响应网斑菌侵染的分子机制，以抗网斑病种质材料BYT-CYA3（B）和敏感型材料美41/I（M）为研究对象，利用转录组测序技术（RNA-Sep），分析了2个材料在接种网斑病菌后不同时间点的基因表达差异。结果表明，对比网斑病菌侵染后不同时间点的转录组测序数据，共鉴定出35 545个差异表达基因；网斑病侵染大麦3 h、6 h、12 h、24 h和72 h时，抗病材料与敏感型材料间富集到GO和KEGG途径的差异表达基因数目有明显区别；共获得435个网斑病菌侵染诱导表达基因；共筛选出182个主要参与过氧化物酶体（peroxisome）、代谢途径（metabolic pathways）、MAPK信号传导途径-植物（MAPK signaling pathway-plant）、植物-病原体相互作用（plant-pathogen）、植物激素信号转导（plant hormone signal transduction）、次生代谢物生物合成（biosynthesis of secondary metabolites）等生物学过程的差异表达基因，推测这些基因与大麦响应网斑病菌侵染有关。随机从中选取10个基因进行了荧光定量PCR检测，并对其转录组测序结果进行定量分析，发现荧光定量PCR结果与转录结果趋势一致。本试验从分子水平初步探索了大麦对网斑病菌侵染的响应机制，为深入研究抗病基因提供了候选基因。 |
| 英文摘要: |
| In recent years,barley net blotch disease caused by the ascomycete fungus Pyrenophora teres has occurred and spread in large areas in the main production area of barley(Hordeum vulgare L.) in China,resulting in a decline in barley yield and quality.In order to explore the molecular mechanism of barley in response to the infection of Pyrenophora teres.In this experiment,the germplasm BYT-CYA3(B) resistant to net blotch disease and the susceptible material Mei 41/I(M) were used as research materials.Transcriptome sequencing was performed to analyze the difference in differentially expressed genes between the two materials at different time points of the spotted pathogen.The results showed that,compared with the transcriptome sequencing data at different time points after infection of Pyrenophora teres,a total of 35 545 differentially expressed genes were identified.The number of differentially expressed genes enriched in GO and KEGG pathway was significantly different between resistant and sensitive materials after 3 h,6 h,12 h,24 h and 72 h infection.A total of 435 genes were obtained,induced expressing by Pyrenophora teres; 182 genes were mainly involved in peroxisome and metabolic pathway,plant MAPK signaling pathway,plant-pathogen interaction,plant hormone signal transduction,and biosynthesis of secondary metabolites.We speculated that these genes are related to barley response to the infection of Pyrenophora teres.Ten genes were randomly selected for real-time quantitative PCR analysis,and the results of transcriptome sequencing were analyzed.It was found that the trend of fluorescence quantitative results was consistent with that of transcriptome sequencing.In this study,the response mechanism of barley to the infection of Pyrenophora teres was explored at the molecular level and provided candidate genes for further study of resistance genes. |
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