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水志杰,安沛沛,刘天相,吴洪启,刘 乐,史 雪,王中华.利用人工合成小麦RIL群体进行小麦穗长和穗宽性状的QTL分析[J].麦类作物学报,2020,(6):656
利用人工合成小麦RIL群体进行小麦穗长和穗宽性状的QTL分析
QTL Analysis of Spike Length and Width UsingRIL Population of Synthetic Wheat
  
DOI:10.7606/j.issn.1009-1041.2020.06.002
中文关键词:  人工合成小麦  RIL群体  穗长  穗宽  QTL
英文关键词:Synthetic wheat  RIL population  Spike length  Spike width  QTL
基金项目:陕西省重点研发农业一般项目(2018NY-056);陕西省自然科学基础研究计划-青年项目(2017JQ3033)
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水志杰,安沛沛,刘天相,吴洪启,刘 乐,史 雪,王中华 (西北农林科技大学农学院陕西杨凌 712100) 
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中文摘要:
      为了挖掘小麦穗长和穗宽性状相关QTL,并为人工合成小麦优异基因资源的开发利用奠定基础,以普通小麦品种西农389×人工合成小麦材料KU98的F7∶8 RIL群体为试验材料,基于小麦55K SNP芯片对F7∶8群体分型结果绘制的遗传连锁图谱及2018和2019年调查统计的F7群体表型数据,对小麦穗长和穗宽性状进行了QTL定位分析。结果表明,在1A、2D、3A、5A和7B染色体上检测到10个与穗长性状相关的QTL,在2D、4D、5A、6A和7D 染色体上检测到10个与穗宽性状相关的QTL,其中与穗长相关的 qSL-2D.1和与穗宽相关的 qSW-4D.1 qSW-5A.1在两年中均被检测到,标记区间分别为AX-111939856~AX-111497351、AX-169335104~AX-110618708、AX-108792246~AX-111048027。 qSW-5A.1连续两年均为主效QTL,且与 qSL-5A.2在同一标记区间,推测该位点具有一因多效的遗传效应或连锁遗传效应。
英文摘要:
      Spike length and width are important spike traits of wheat,which are closely related to yield. In this study,the F7∶8 RIL population constructed by crossing the synthetic wheat KU98 with the common wheat Xinong 389 was used for mapping. F7 individuals were genotyped by wheat 55K SNP array and spike phenotypes were investigated and analyzed in 2018 and 2019. QTL mapping program was used to generate high-density linkage map and mapping analysis. Ten QTLs related to spike length were detected on chromosomes 1A,2D,3A,5A and 7B,and ten spike width-related QTLs were detected on chromosomes 2D,4D,5A,6A and 7D. Spike length-related locus qSL-2D.1 and spike width-related loci qSW-4D.1 and qSW-5A.1 were consistent in 2018 and 2019,whose location intervals were AX-111939856-AX-111497351,AX-169335104-AX-110618708 and AX-108792246-AX-111048027,respectively. The major QTL qSW-5A.1 was located in the same interval as qSL-5A.2 which is speculated as a multi-effect or interlocking genetic effect. This study laid a foundation for the exploration of QTLs related to spike length and width and for the excellent gene resources of synthetic wheat.
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