| 郭双元，任惠文，张艳琴，冯传欣，冯 浩，王晓杰，康振生，张新梅.小麦抗病相关基因 TaTGA2.2在小麦-条锈菌互作中的表达分析及功能研究[J].麦类作物学报,2020,(6):645 |
| 小麦抗病相关基因 TaTGA2.2在小麦-条锈菌互作中的表达分析及功能研究 |
| Characterization and Functional Analyses of Wheat Disease Resistance-Related Gene TaTGA2.2 in the Interaction Between Wheat and Stripe Rust |
| |
| DOI：10.7606/j.issn.1009-1041.2020.06.01 |
| 中文关键词: 小麦条锈菌 碱性亮氨酸拉链 亚细胞定位 VIGS |
| 英文关键词:Puccinia striiformis f. sp. tritici Basic leucine zipper Subcellular localization VIGS |
| 基金项目:陕西省自然科学基础研究计划项目(2018JM3031)；高等学校学科创新引智计划资助项目（B07049） |
|
| 摘要点击次数: 612 |
| 全文下载次数: 633 |
| 中文摘要: |
| 为了进一步解析小麦与条锈菌（Puccinia striiformis f. sp. tritici，Pst）互作中的抗病分子机制，在小麦-条锈菌互作的cDNA文库中分离得到一个编码bZIP类转录因子的小麦抗病相关基因 TaTGA2.2。通过实时荧光定量PCR（Quantitative real-time PCR，qRT-PCR）以及病毒诱导的基因沉默技术（VIGS）对 TaTGA2.2的表达模式及其在小麦与条锈菌互作中的功能进行了初步解析。结果表明，小麦 TaTGA2.2基因全长1 005 bp，编码334个氨基酸，具有bZIP保守结构域以及TGA转录因子类似结构域。进化树分析发现TaTGA2.2与一粒小麦中TGA蛋白近缘。拟南芥原生质体亚细胞定位发现 TaTGA2.2分布在细胞核，酵母自激活实验表明 TaTGA2.2蛋白N端具有转录激活活性。此外， TaTGA2.2受条锈菌诱导表达，且非亲和互作中的表达量较亲和互作明显增高。非生物胁迫处理中干旱、盐以及外源激素水杨酸（SA）和脱落酸（ABA）也能诱导 TaTGA2.2上调表达。VIGS沉默 TaTGA2.2基因后，发现接种条锈菌CYR23及CYR31后小麦的表型均无明显变化。另外，PR基因的表达量也无显著差异，表明植物中可能存在与 TaTGA2.2功能冗余的TGA成员。 |
| 英文摘要: |
| In order to further analyze the molecular mechanism of disease resistance in the interaction between wheat and Puccinia striiformis f. sp. tritici(Pst), a wheat disease-resistance gene TaTGA2.2, encoding a bZIP transcription factor, was isolated from the cDNA library of wheat-Pst interaction. Quantitative real-time PCR (qRT-PCR) and virus-induced gene silencing (VIGS) were used to preliminarily analyze the expression characteristics and function of TaTGA2.2. This study focused on the TaTGA2.2, which contains a 1 005 bp open reading frame, encoding one class of bZIP transcription factor with 334 amino acids. Phylogenetic analysis revealed that TaTGA2.2 had the closest evolutionary distance from TmTGA.Arabidopsis protoplasts subcellular localization analysis found that TaTGA2.2 is distributed in the nucleus. Transcriptional activation analysis in yeast revealed that TaTGA2.2 exhibits transcriptional activity in its N-terminal. The level of TaTGA2.2 transcripts were up-regulated induced by Pst as well as drought and salt, but the level of expression was apparently higher in incompatible interaction than that in compatible interaction. Besides, the transcription of TaTGA2.2 was also induced by exogenous hormone SA and ABA. When knocked down TaTGA2.2 with VIGS system, there was no significant difference in phenotype, and the expression level of PR genes also did not change significantly. The results indicate that functional redundant TGA members with TaTGA2.2 may exist in wheat. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
基本信息
