| 栾海业，徐 肖，陶 红，臧 慧，张英虎，乔海龙，杨红燕，陈 健，陈 和，沈会权.大麦EMS突变体库构建和突变体筛选[J].麦类作物学报,2020,(5):576 |
| 大麦EMS突变体库构建和突变体筛选 |
| Construction of EMS-Treated Mutant Library and Mutant Phenotypic Analysis in Barley |
| |
| DOI：10.7606/j.issn.1009-1041.2020.05.08 |
| 中文关键词: 大麦 EMS诱变 突变体 |
| 英文关键词:Barley EMS inducement Mutant |
| 基金项目:国家大麦青稞产业技术体系项目(CARS-05)；江苏省农业自主创新项目［CX(17)3056］；江苏农业科技援藏-青稞种质资源鉴定及新品系选育项目［JATS(2018)139］；上海市科技兴农项目（2019-02-08-00-08-F01109） |
|
| 摘要点击次数: 770 |
| 全文下载次数: 863 |
| 中文摘要: |
| 为研究大麦重要功能基因并给大麦遗传育种提供新的种质资源，通过甲基磺酰乙酯（EMS）诱变处理优质、高抗黄花叶病大麦品种苏啤6号，从2 400个M2代株系中筛选出152个突变株系，突变频率为 6.33%。其中，幼苗习性突变株系36个，突变率为1.50%；叶部突变株系34个，突变率为1.42%；茎部突变株系33个，突变体率为1.38%；穗部突变株系22个，突变率为0.92%；成熟期和育性突变株系27个，突变率为1.13%。经过M3代验证，获得可稳定遗传的各类突变株系43个。 |
| 英文摘要: |
| In order to provide basic materials for studying the functional genomics and genetic breeding of barley,Supi 6 with a high quality and resistance to yellow mosaic disease was treated with the chemical mutagen ethyl methane sulfonate(EMS). A total of 152 mutants with significantly visible phenotypes were screened out from the 2 400 M2 generation,with a mutation frequency of 6.33%. Of them,36 lines varied in seedling habits,with a mutation frequency of 1.50%; 34 lines varied in leaf,with a mutation frequency of 1.42%; 33 lines varied in stalk,with a mutation frequency of 1.38%; 22 lines varied in spike,with a mutation frequency of 0.92%; 27 lines varied in maturation stage and fertility,with a mutation frequency of 1.13%. In total,43 stably inherited lines were obtained by further agronomic trait investigation in M3 generation. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
基本信息
