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白海波,吕学莲,惠 建,马斯霜,李树华,董建力.春小麦抗旱耐热性QTL分析[J].麦类作物学报,2020,(3):300
春小麦抗旱耐热性QTL分析
QTL Analysis for Drought and Heat Resistance of Spring Wheat
  
DOI:10.7606/j.issn.1009-1041.2020.03.06
中文关键词:  春小麦  抗旱  耐热  QTL定位  分析
英文关键词:Wheat  Drought resistance  Heat resistant  QTL mapping  Analysis
基金项目:国家自然科学基金项目(31460345,31960428);宁夏回族自治区自然科学基金项目(2018AAC03189);宁夏青年科技人才托举工程项目(TJGC2018025);宁夏回族自治区农业育种专项(2018NYYZ0202);宁夏农林科学院科技创新先导资金项目(NKYJ-17-24)
作者单位
白海波,吕学莲,惠 建,马斯霜,李树华,董建力 (宁夏农林科学院农业生物技术研究中心宁夏银川 750002) 
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中文摘要:
      为发掘控制小麦抗旱耐热基因位点,以小麦重组自交系(RILs)群体为材料,于2015-2017年小麦灌浆期进行干旱胁迫、热胁迫、旱热胁迫处理,并对抗旱耐热相关性状QTL进行分析。结果表明,在干旱胁迫、热胁迫、旱热胁迫下分别检测到22、36和30个QTL,其中控制旗叶叶绿素含量、旗叶含水量、穗粒重、千粒重的QTL数分别为26、21、22和19个。抗旱相关性状QTL位于2A、3B、4B、7B、3D、4D和7D染色体上,表型贡献率为9.38%~30.81%;耐热相关性状QTL位于2A、2B、3A、3B、4B、4D、5D、6D、7A和7B染色体上,表型贡献率为9.03%~34.97%。抗旱性共定位区间2个,分别位于2A(gwm294-wmc644)和7B(barc140-gwm297)染色体上;耐热性共定位区间2个,分别位于5D(cfd67-cfd40)和6D( barc196-barc54)染色体上;抗旱耐热性共定位区间3个,分别位于2A(gwm294-wmc644)、2B(wmc441-wmc317)和7B(wmc83-wmc276)染色体上,其中共定位区间 gwm294-wmc644( Qcc-2A.2 Qgw-2A.1)的贡献率超过10%,遗传距离较小(6.49 cM)。
英文摘要:
      In order to find out the gene loci controlling drought and heat resistance in wheat, QTL(quantitative trait locus) analysis was carried out in wheat under drought stress, heat stress and drought + heat stresses during grain filling stage using RILs(recombinant inbred lines) from 2015 to 2017. The results showed that 22,36 and 30 QTLs were detected under drought stress, heat stress and drought + heat stresses, and 26, 21, 22 and 19 QTLs were related to leaf chlorophyll content, relative water content, grain weight and thousand grain weight. QTLS for drought-related traits were located on chromosomes 2A, 3B, 4B, 7B, 3D, 4D and 7D with the phenotypic contribution rate ranging from 9.38%to 30.81%. QTLS for heat-resistant traits were located on chromosomes 2A, 3A, 7A, 3B, 4B, 7B, 4D, 5D and 6D with the phenotypic contribution rate ranging from 9.03% to 34.97%. Two co-localization intervals were located on chromosomes 2A(gwm294-wmc644)and 7B(barc140-gwm297) under drought treatment; two co-localization intervals were located on chromosomes 5D (cfd67-cfd40) and 6D ( barc196-barc54)under drought treatment, and three co-localization intervals were located on chromosome 2A (gwm294-wmc644), 2B (wmc441-wmc317) and 7B (wmc83-wmc276)under drought + heat treatment. The contribution rates of the co-localization interval gwm294-wmc644( Qcc-2A.2 and Qgw-2A.1) were over 10% and the interval distance was 6.49 cM.
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