| 朱冬梅,胡文静,别同德,陆成彬,赵仁慧,高德荣.利用四交RIL群体定位小麦籽粒脱水速率QTL[J].麦类作物学报,2020,(1):49 |
| 利用四交RIL群体定位小麦籽粒脱水速率QTL |
| QTL Mapping for Kernel Dehydration Rate after Physiological Maturity Using Four-Way RIL Populations of Wheat |
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| DOI：10.7606/j.issn.1009-1041.2020.01.06 |
| 中文关键词: 小麦 四交重组自交系 脱水速率 数量性状基因位点 |
| 英文关键词:Wheat Four-way wheat RIL population Kernel dehydration rate QTL |
| 基金项目:国家自然科学基金项目(31901544)；国家现代农业产业技术体系建设专项(CARS-03-03B，CARS-3-2-11)；国家重点研发计划项目(2017YFD0100801，2016YFD0101802)；江苏省自然科学基金项目(BK20171279) |
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| 中文摘要: |
| 为了探索小麦生理成熟后籽粒脱水速率的遗传机制，以扬麦16、镇麦168、扬麦20和扬麦22为亲本构建四亲本RIL群体，利用小麦15K SNP芯片构建其遗传连锁图谱，并对小麦籽粒脱水速率QTL进行定位。结果共检测到12个与小麦籽粒脱水速率相关的QTL，分布在1AL(2)、2AL、3BS、3BL、4AS、5BL、6DL、7AL、7BS、7BL和7DS，其中 QDR-yaas-6DL、 QDR-yaas-1AL.1和 QDR-yaas-3BL脱水速率增效基因仅来自扬麦16，可分别解释表型变异的8.1%、7.6%和2.8%； QDR-yaas-1AL.2， QDR-yaas-2AL， QDR-yaas-4AS和 QDR-yaas-7DS脱水速率增效基因仅来自镇麦168，可解释表型变异的3.6%~4.2%； QDR-yaas-5BL脱水速率增效基因仅来自扬麦20，可解释表型变异的5.4%； QDR-yaas-3BS和 QDR-yaas-7BS脱水速率增效基因来自扬麦20和扬麦16，可解释表型变异的3.2%和3.8%； QDR-yaas-7AL和 QDR-yaas-7BL脱水速率增效基因来自镇麦168和扬麦16，可解释表型变异的4.8%和5.9%。推测扬麦16、镇麦168脱水速率快的特性遗传于扬麦158。本研究结果将为小麦生理成熟后籽粒脱水性状的深入研究奠定基础。 |
| 英文摘要: |
| To study the genetic mechanism of the kernel dehydration rate after physiological maturity,a four-way RIL population constructed with ZM168,YM20,YM16 and YM22 as parents was genotyped with wheat 15K SNP array to identify the QTL associated with kernel dehydration rate. A total of 12 QTLs were identified through software GAPL(http://www.isbreeding.net),which were located on chromosomes 1AL(2)，2AL，3BS，3BL，4AS，5BL，6DL，7AL，7BS，7BL and 7DS,respectively. QDR-yaas-6DL, QDR-yaas-1AL.1 and QDR-yaas-3BL were derived from YM16,which explained 8.1%,7.6% and 2.8% of phenotypic variation,respectively. QDR-yaas-1AL.2, QDR-yaas-2AL, QDR-yaas-4AS and QDR-yaas-7DS explaining 3.6%-4.2% of phenotypic variation were derived from ZM168. YM20 carrying QDR-yaas-5BL explained 5.4% of phenotypic variation. QDR-yaas-3BS and QDR-yaas-7BS were derived from YM20 and YM16,explaining 3.2% and 3.8% of phenotypic variation. QDR-yaas-7AL and QDR-yaas-7BL existed in ZM168 and YM16 simultaneously,which explained 4.8% and 5.9% of phenotypic variation. Analysing the pedigree of YM16 and ZM168,we found that the high dehydration rate may be inherited from Yangmai 158. The QTLs identified in this study will lay a foundation for further research on the kernel dehydration rate after physiological maturity. |
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