| 刘晓宇,吴洪启,石善党,李春莲,汪 勇,王中华.小麦表皮蜡质脂肪醇合成基因 TaFAR10的克隆及功能验证[J].麦类作物学报,2020,(1):21 |
| 小麦表皮蜡质脂肪醇合成基因 TaFAR10的克隆及功能验证 |
| Cloning and Functional Analysis of Epidermal Wax Fatty Alcohol Synthesis Gene TaFAR10 in Common Wheat |
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| DOI：10.7606/j.issn.1009-1041.2020.01.003 |
| 中文关键词: 小麦 蜡质 初级醇 TaFAR10 酵母表达 表达分析 |
| 英文关键词:Wheat Cuticular waxes Primary alcohol TaFAR10 Yeast expression Expression analysis |
| 基金项目:国家自然科学基金项目（31471568）；陕西省重点科技创新团队项目（2014KCT-25）；中国博士后科学基金面上项目（2016M602862）；中国博士后科学基金特别资助项目(2018T111107） |
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| 中文摘要: |
| 在植物的生长发育中，植物表皮蜡质能够保护植物免受外来生物和非生物胁迫的侵害。 TaTAA1a编码脂肪酰基辅酶A还原酶，在花药绒毡层细胞中该基因有助于脂肪醇物质的产生。为探究 TaTAA1a是否参与小麦叶片表皮蜡质成分中初级醇的生物合成，本研究从低蜡小麦品系CP98（11）中克隆了 TaTAA1a基因，并将其命名为 TaFAR10。 TaFAR10的开放阅读框为1 524 bp，编码一个具有507个氨基酸的蛋白。序列比对发现， TaFAR10与 TaFAR4、 TaTAA1c、 TaFAR5具有相似的功能结构域。系统进化树分析表明， TaFAR10、 TaFAR4、 TaTAA1c亲缘关系较近，并且与拟南芥的 AtFAR4聚为一类。酵母异源表达结果显示， TaFAR10具有催化C18:0脂肪醇合成的功能。另外 TaFAR10呈现组织特异性表达，在旗叶、苗期叶片、叶鞘、穗下茎、颖壳、茎节、芒、花药和根中均表达，其中，在旗叶和苗期叶片中表达量最高。在ABA、干旱、PEG、冷胁迫条件下， TaFAR10的表达量呈下降趋势，表明 TaFAR10不能受非生物胁迫诱导。该研究为深入了解小麦表皮蜡质脂肪醇合成的分子机制具有重要的参考价值。 |
| 英文摘要: |
| During plants growth and development,cuticular waxes can protect plants from exotic biotic and abiotic stresses. TaTAA1a encodes a fatty acyl coenzyme A reductase,which contributes to the production of fatty alcohols in anther tapetum cells. In order to study whether the gene is involved in the biosynthesis of primary alcohols of wheat leaf cuticular waxes. In this study,we cloned gene TaTAA1a from a low wax strain CP98(11) and renamed TaFAR10. The ORF length of TaFAR10 is 1 524 bp ,encoding a protein of 507 amino acids. Sequence alignment revealed that TaFAR10 has similar functional domains with TaFAR4, TaTAA1c and TaFAR5. Phylogenetic tree analysis showed that TaFAR10 were closely related to TaFAR4 and TaTAA1c and they were closely related to AtFAR4. The result of heterologous expression in yeast showed that TaFAR10 had the function of synthesizing C18:O-OH alcohol. In addition, TaFAR10 gene also expressed in flag leaf,seedling leaves,sheath,peduncle,glume,stem,arista,anther and root and highest expression in flag leaves and seeding leaves. Moreover,the expression of TaFAR10 gene showed a downregulated trend under ABA ,drought,PEG and cold stress. The results of this study laid the foundation for further study on the molecular mechanism of wheat epidermal wax fatty alcohol synthesis. |
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