| 李孟军，周 硕，李亚青，张士昌，彭义峰，张 楠，史占良.普通小麦部分同源染色体配对抑制基因 Ph1分子标记的验证和筛选[J].麦类作物学报,2019,(12):1393 |
| 普通小麦部分同源染色体配对抑制基因 Ph1分子标记的验证和筛选 |
| Validation and Screening of Molecular Markers of Ph1 Gene in Common Wheat |
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| DOI：10.7606/j.issn.1009-1041.2019.12.01 |
| 中文关键词: 小麦 抑制部分同源染色体配对基因（Ph1） ph1b突变体 分子标记 |
| 英文关键词:Wheat Ph1 ph1b mutant Molecular marker |
| 基金项目:国家重点研发计划项目（2017YFD0100601）；河北省农林科学院创新工程课题(2019-4-8-1)；石家庄市科学技术研究与发展计划项目(91490332A) |
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| 中文摘要: |
| 在ph1b纯合基因型中，普通小麦部分同源染色体和外源染色体能够发生配对和重组。为通过分子标记辅助育种技术高效利用ph1b突变体进行异源有益基因转移，以11个小麦材料为试材，比较了9个已报道的Ph1的PCR分子标记。在9个分子标记中，标记OPR7和OPR17不具有特异性，标记PhSCAR、Mads、Ph920、PSR128、PSR574、PSR2120和WPG90为显性标记，仅标记PhSCAR为共显性标记。结果表明，标记Mads、PSR128、PSR574和PhSCAR扩增稳定，条带清晰，无非特异性PCR扩增产物，是用于Ph1分子检测的理性标记。 |
| 英文摘要: |
| In homozygous ph1b genotypes, chromosome pairing and recombination occurs between homeologous and alien chromosomes in wheat. In order to transfer alien genes efficiently by molecular marker assistant selection, nine molecular markers of Ph1 were compared by using 11 wheat varieties. Among the nine PCR markers, two markers(OPR7 and OPR17), had non-specific amplicons between ph1b mutants and wild type.Seven markers(PhSCAR,Mads, Ph920, PSR128, PSR574, PSR2120 and WPG90) were dominant PCR markers,but only marker PhSCAR was a co-dominant PCR marker. The results showed four markers(Mads, PSR128, PSR574 and PhSCAR) were specific PCR markers for Ph1 with no non-specific amplicons,which were recommended to be used in wheat molecular assistant selection breeding for homozygous ph1b genotypes. |
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