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李 聪,马 建,刘 航,丁浦洋,杨聪聪,张 涵,秦娜娜,兰秀锦.基于小麦55K SNP芯片检测小麦穗长和株高性状QTL[J].麦类作物学报,2019,(11):1284
基于小麦55K SNP芯片检测小麦穗长和株高性状QTL
Detection of QTLs for Spike Length and Plant Height in Wheat Based on 55K SNP Array
  
DOI:10.7606/j.issn.1009-1041.2019.11.03
中文关键词:  普通小麦  穗长  株高  QTL  小麦55K SNP芯片
英文关键词:Common wheat  Spike length  Plant height  QTL  Wheat 55K SNP array
基金项目:本研究由四川省国际合作交流项目基金(2017HH0076)项目资助
作者单位
李 聪,马 建,刘 航,丁浦洋,杨聪聪,张 涵,秦娜娜,兰秀锦 (四川农业大学小麦研究所四川成都 611130) 
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中文摘要:
      为了进一步挖掘小麦穗长和株高的QTL位点,将小麦品系‘20828’和国审小麦品种川农16杂交后得到的重组自交系群体(RIL)于2016-2018年种植在四川崇州、温江和雅安以及孟加拉国库尔纳市,进行穗长和株高的遗传分析。结果表明,在RIL群体内,穗长和株高均呈正态分布,符合数量性状遗传特点。在3年8个环境中共检测出44个控制穗长和株高的QTL,其中稳定的穗长QTL有5个,分布于2D、4B和5B染色体上,贡献率为2.90%~26.26%;控制株高的稳定QTL有3个,分布于2D、4B和4D染色体上,贡献率为1.96%~21.22%。在2D染色体AX-86171316~AX-109422526标记区间和4B染色体AX-109526283~[JP]AX-110549715标记区间均同时检测到了控制穗长和株高的QTL,推测为一因多效位点。QSl.sau.2D.1QSl.sau.2D.2QSl.sau.4B.1 QSl.sau.4B.2 QSl.sau.5B QPh.sau.2D QPh.sau.4B可能为新的稳定QTL。本研究鉴定的这些位点对增加穗长、降低株高以及提高小麦的产量具有重要作用。
英文摘要:
      In order to further excavate QTLs for spike length and plant height of wheat,a recombinant inbred line(RIL) population obtained from the cross between a wheat line ‘20828’ and a national authorized wheat variety ‘Chuannong 16’ was planted and evaluated in Wenjiang,Chongzhou,and Ya’an of Sichuan from 2016 to 2018 and Khulna Bgladesh in 2018. The spike length and plant height showed continuous variation with approximately normal distributions,indicating that they are suitable for QTL analysis. A total of 44 QTLs were detected in the eight environments. Five stable QTLs associated with spike length were detected on chromosomes 2D,4B and 5B,explaining 2.90% to 26.26% of phenotypic variations. Three stable QTLs associated with plant height were detected on chromosomes 4B,4D and 2D,explaining 1.96% to 21.22% of phenotypic variations. The loci located in the interval of AX-86171316-AX-109422526 on chromosome 2D and AX-109526283-AX-110549715 on chromosome 4B controlled both spike length and plant height and may be a pleiotropic QTL. Furthermore, QSl.sau.2D.1, QSI.sau.2D.2, QSl.sau.4B.1, QSl.sau.4B.2, QSl.sau.5B, QPh.sau.2D, QPh.sau.4B and may be novel stable QTLs. These detected loci will be useful for increasing spike length,decreasing plant height and boosting grain yield.
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