| In order to accurately evaluate the ecological safety of the wheat chemical hybridization agent SQ-1,this study used wheat treated with 5 kg·hm-2 SQ-1 and control wheat(treated with an equal amount of water) as food for the grain aphid Sitobion avenae.They were fed for 11 consecutive generations,and the life table data of the 2nd,6th,and 11th generations of aphids were recorded.At the same time,total enzyme solution of the 2nd,6th,and 11th generations of aphids were extracted from the treatment group and the control group of aphids.In addition,the enzymatic activity of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),and the detoxification enzyme acetylcholinesterase(AchE) was measured.The results showed that population parameters(intrinsic rate of increase rm,finite rate of increase λ,net reproductive rate R0,and mean generation time T) were decreased with the increase of generation.Among population parameters,the intrinsic rate of increase rm,finite rate of increase λ,and net reproductive rate R0 in the treatment group in the 6th generation were significantly lower than those in the control group(P<0.05),while those in the 2nd and 11th generations were not significantly different from those in the control group.There was no significant difference in mean generation time T between the treatment group and the control group of each generation of aphids.Meanwhile,the trend of the total pre-oviposition period TPOP and longevity in the treatment group were consistent with those in the control group,and the fecundity parameters(total pre-oviposition period TPOP,oviposition days and longevity) in the treatment group was not significantly different from the control group in all generations.Enzyme activity measurements showed that CAT activity in the treatment group was not significantly different from that in control group in 6th generation,while that in the 2nd and 11th generations was significantly higher,compared to the control group(P<0.05).Meanwhile,the activities of SOD,POD and AchE in the treatment group in all generations were significantly higher than those in the control group(P<0.05).With the increase of the generations,the SOD activity in the treatment group showed a decreasing trend,while that in the control group first rose and then declined.The POD activity first increased and then decreased in the treatment group,but first decreased and then increased in the control group.The CAT activity first fell and subsequently increased with the increase of the generation in treatment group,and furthermore,it showed a declining trend in the control group.Contrary to the above enzyme,the activity of the detoxification enzyme AchE was increased at the early stage and then decreased both in treatment group and control group.There was a positive correlation between SOD,POD,CAT and AchE activity.These results indicate that the synergistic work of the protective enzyme system and the detoxification metabolic pathway are important to ensure that the population parameters return to the normal level with the increase of generation and fecundity parameters do not vary significantly under SQ-1 stress.Furthermore,the biological self-protective enzyme system and the detoxification pathway can repair the stress damage caused by certain doses of the chemical hybridization agent SQ-1.Therefore,it is believed that the wheat chemical hybridization agent SQ-1 has some degree of ecological safety. |
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