| 袁国徽，李 涛，钱振官，田志慧，刘伟堂，王金信.抗精噁唑禾草灵耿氏硬草乙酰辅酶A羧化酶基因研究[J].麦类作物学报,2019,(8):928 |
| 抗精噁唑禾草灵耿氏硬草乙酰辅酶A羧化酶基因研究 |
| Study of the Acetyl-CoA Carboxylase Gene of Sclerochloa kengiana Populations with Resistance to Fenoxaprop-P-ethyl |
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| DOI：10.7606/j.issn.1009-1041.2019.08.06 |
| 中文关键词: 耿氏硬草 精噁唑禾草灵 抗药性 基因突变 乙酰辅酶 A羧化酶 |
| 英文关键词:Sclerochloa kengiana Fenoxaprop-P-ethyl Herbicide-resistance Gene mutation Acetyl-CoA carboxylase |
| 基金项目:国家重点研发计划项目(2018YFD0200500）；国家自然科学基金项目(31772181） |
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| 中文摘要: |
| 为明确耿氏硬草抗性种群SD-23对乙酰辅酶A羧化酶（ACCase）抑制剂类除草剂的抗性水平及靶标抗性分子机理，采用整株水平测定法测定了SD-23种群对6种ACCase抑制剂类除草剂的抗性，扩增和比对了抗性和敏感种群间的ACCase基因序列，并测定了耿氏硬草种群SD-23对其他4种除草剂的敏感性。结果表明，SD-23种群对精噁唑禾草灵产生了高水平抗性，抗性倍数为12.7，对炔草酯和精吡氟禾草灵产生了低水平抗性，抗性倍数分别为5.2和4.5，对烯禾啶、烯草酮和唑啉草酯较敏感。与敏感种群SD-1相比，SD-23种群ACCase的CT区域第2096位甘氨酸突变为丙氨酸（Gly-2096-Ala）；甲基二磺隆、啶磺草胺和异丙隆对抗性和敏感种群均有较好防效，其防效达86.7%以上，干重防效达77.4%以上。ACCase的Gly-2096-Ala突变第一次在耿氏硬草中被报道，该突变可能是导致SD-23种群对精噁唑禾草灵产生抗性的重要原因之一。 |
| 英文摘要: |
| The objective of this study is to investigate the resistance levels of Sclerochloa kengiana population(SD-23) to acetyl-CoA carboxylase(ACCase)-inhibiting herbicides and its molecular basis of resistance. Whole-plant dose-response assays demonstrated that SD-23 population showed a high-level resistance to fenoxaprop-P-ethyl with a resistance index of 12.7,and a low-level resistance to clodinafop-propargyl and fluazifop-P-butyl,with resistance indices of 5.2 and 4.5,respectively,and was susceptible to sethoxydim,clethodim and pinoxaden.By comparing the sequences derived from the resistant and susceptible populations,the SD-23 population was found to contain a glycine-to-alanine mutation at the 2 096th bp of ACCase coding region.Simultaneously,whole-plant pot assays were conducted to evaluate the susceptibilities to other four herbicides in the resistant and susceptible populations.The results showed that the herbicides including mesosulfuron-methyl,pyroxsulam and isoproturon,had high efficacy against the S.kengiana populations,with a plant control efficiency over 86.7% and a dry weight control efficiency over 77.4%.The Gly-2096-Ala mutation in the ACCase gene was one of main reasons for the resistance to fenoxaprop-P-ethyl in the SD-23 population,which is firstly reported in S.kengiana. |
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