A field experiment was conducted to study the impact of biocharaddition on photosynthetic potential and grain yield of winter wheat. Four treatments, including 7 200 kg·hm-2(BH), 3 600 kg·hm-2(BM),1 800 kg·hm-2(BL) and 0 (BO) kg·hm-2 biochar addition rates, were conducted at Wuqiao Experimental Station of China Agricultural University, Hebei Province. We measuredthe diurnal variation and light-response of net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, and transpiration rate of winter wheat flag leavesat anthesis and grain-filling stages. The results showed that the diurnal variation of net photosynthetic rate, stomatal conductance and transpiration rate of flag leaf in the anthesis and grain-filling stages of winter wheat showed a bimodal curve under different biochar addition rates with obvious photosynthetic “noon break”, while the biochar addition could weaken the “noon break”.The diurnal variation of intercellular CO2 concentration of winter wheat leaf decreased at first and then increased, presentinga “V-shape” under all treatments.With the increase of light intensity, the light-response curves of net photosynthetic rate, stomatal conductance and transpiration rate showed a gradual upward trend at the anthesisstage, while the response curves of the grain-filling stage showed a gradually downward trend when the light intensity was 1 600-2 000 μmol·m-2·s-1. In terms of different treatments, BH and BM were higher than BL and BO in the anthesis and grain-filling stages.The simulated maximum net photosynthetic rate of grain-filling stage was treated with BM, which was higher than BH, BL and BO by 2.50%, 6.20%, and 8.88%, respectively.Compared with BO, the grain yield of BH, BM and BL increased by 4.14%, 5.97%, and 1.49%, respectively(P<0.05). Therefore, biochar addition can weaken the photosynthesis “noon break”at the anthesis and grain-filling stages, enhance the photosynthetic performance andpotential, and improve the grain yield of winter wheat. It is more suitable to apply the amount of 3 600 kg·hm-2 per year under the condition the same with this experiment. |