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作者	单位
王炜 ,陈琛 ,叶春雷,杨随庄,欧巧明,罗俊杰	（1.甘肃省农业科学院生物技术研究所，甘肃兰州 730070；2.西南科技大学生命科学与工程学院,四川绵阳 621010）

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中文摘要:

为快速获得携带麦谷蛋白优质亚基基因的小麦新品种，提高小麦的品质育种技术水平，利用引进的矮败材料与和尚头、甘春20号、临麦34号等10个不同品种（系）杂交，并对杂交后代进行了花药培养，获得了115份花培株系；利用PCR对花培后代株系及杂交亲本进行了优质贮藏蛋白亚基分子标记检测，3个HMW-GS为Bx7、Bx14、Dx5，3个LMW-GS为Glu-A3ac、Glu-A3d、Glu-B3b。结果表明，在115份花培材料中，Bx7的出现频率最高，为94.78%，其余依次为Glu-A3ac、Dx5、Bx14、Glu-A3d和Glu-B3b；获得了44份聚合4个亚基以上的材料；结合农艺性状鉴定，筛选出了3份综合性状优异的小麦新品系AB158、AB167和AB332。本研究将花培育种技术、分子标记辅助选择技术及矮败小麦育种技术进行了有机结合，其结果可为提升小麦品质育种技术水平提供参考。

英文摘要:

To breed wheat new variety with elite glutenin subunits and improve the technical level of breeding for wheat quality improvement, the hybrid progenies of an introduced dwarf-sterile wheat crossed with 10 different varieties, such as Heshangtou, Ganchun 20, Linmai 34, etc. were anther-cultured and 115 anther-cultured plant lines were obtained, which were identified by using three HMW-GS(Bx7, Bx14, and Dx5) and three LMW-GS(Glu-A3ac, Glu-A3d, and Glu-B3b) molecular markers to determine the presence of these subunits in the anther-cultured progenies and the hybrid parents. Results showed that the frequency of Bx7 is the highest among these 115 materials, accounting for 94.78%, followed by the remaining alleles:Glu-A3ac, Dx5, Bx14, Glu-A3d and Glu-B3b; 44 materials contain at least four subunits; three new wheat lines with elite comprehensive traits were selected by combining with determination of agronomic traits in the field. The anther-cultured breeding technology, molecular marker technology, and dwarf-sterile wheat breeding technology were combined in this research, and the results can provide reference for improving the technical level of wheat quality breeding.

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