| 谢 磊，战帅帅，王丽丽，任 毅，时 佳，耿洪伟.新疆冬小麦过氧化物酶基因TaPod-D1和TaPod-A1的等位变异及分布[J].麦类作物学报,2019,(3):262 |
| 新疆冬小麦过氧化物酶基因TaPod-D1和TaPod-A1的等位变异及分布 |
| Molecular Identification and Distribution Analysis of Peroxidase Gene TaPod-D1 and TaPod-A1 in Xinjiang Winter Wheat |
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| DOI：10.7606/j.issn.1009-1041.2019.03.02 |
| 中文关键词: 新疆冬小麦 过氧化物酶基因 等位变异检测 分子标记 |
| 英文关键词:Xinjiang winter wheat Peroxidase activity gene Allelic variation detection Molecular marker |
| 基金项目:国家自然科学基金项目(31771786)；新疆自治区青年科技创新人才培养工程项目（QN2016JQ0025） |
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| 中文摘要: |
| 为了给新疆小麦品种面粉色泽的改良提供参考依据，利用位于7D染色体上的功能标记POD-7D1和POD-7D6及3A染色体上的功能标记POD-3A1和POD-3A2对98份新疆冬小麦品种（系）进行等位变异检测。结果表明，在TaPod-D1位点，有42份（占42.9%）材料含有与高POD活性相关的TaPod-D1a等位基因，56份（占57.1%）材料含有与低POD活性相关的TaPod-D1b等位基因；在TaPod-A1位点，有28份（占28.6%）材料含有与高POD活性相关的TaPod-A1b等位基因，70份（占71.4%）材料含有与低POD活性相关的TaPod-A1a等位基因。在不同类型的新疆冬小麦品种（系）中，两个不同位点上高POD活性等位变异TaPod-D1a和TaPod-A1b的分布频率均表现为引进品种（系）≈自育品种（系）＞地方品种。所检测的新疆冬小麦品种（系）共有四种等位基因组合，分别为Tapod-D1b/Tapod-A1a、Tapod-D1a/Tapod-A1a、 Tapod-D1b/Tapod-A1b和Tapod-D1a/Tapod-A1b，它们在新疆冬小麦品种（系）中的分布频率依次为39.8%、31.6%、17.4%和11.2%。 |
| 英文摘要: |
| In order to provide crucial information and methods for flour color improvement in wheat breeding program, 98 Xinjiang winter wheat varieties(lines) were detected by functional markers POD-7D1 and POD-7D6, POD-3A1 and POD-3A2 on 7D and 3A chromosomes, and the distribution patterns of different alleles of these genes encoding the peroxidase(peroxidase, POD) were analyzed. The results showed that 42(42.9%) out of the 98 Xinjiang winter wheat varieties(42.9%) containTaPod-D1a allele associated with high POD activity, and 56(57.1%) containTaPod-D1b allele associated with low POD activity. At theTaPod-A1 locus, 28(28.6%) materials containTaPod-A1b allele associated with high POD activity, and 70(71.4%) materials containTaPod-A1a allele associated with low POD activity. In different types of Xinjiang winter wheat varieties(lines), the introdused varieties had the highest frequencie of the genotypeTaPod-D1a and TaPod-A1b followed by self-cultivated varieties and local varieties. The genotype combinationsTapod-D1b/Tapod-A1a， Tapod-D1a/Tapod-A1a，Tapod-D1b/Tapod-A1b andTapod-D1a/Tapod-A1b were the most popular genotypes in Xinjiang winter wheat varieties with frequencies of 39.8%,31.6%,17.4%,and 11.2%, respectively. |
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