| 李梦达，左 宁，杜红旭，靳海洋，贺德先，牛洪斌.小麦 TaSABP2基因的克隆及表达分析[J].麦类作物学报,2019,(2):140 |
| 小麦 TaSABP2基因的克隆及表达分析 |
| Cloning and Expression Analysis of TaSABP2 Gene in Wheat |
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| DOI：10.7606/j.issn.1009-1041.2019.02.03 |
| 中文关键词: 小麦 TaSABP2基因 基因克隆 生物信息学 表达分析 |
| 英文关键词:Wheat (Triticum aestivum L.) TaSABP2 Gene cloning Bioinformatics Expression analysis |
| 基金项目:国家“十二五”科技支撑计划项目（2013BAD07B07-4）；国家“十三五”重点研发计划项目（2017YFD0301101） |
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| 中文摘要: |
| 为挖掘小麦抗逆基因，进一步解析小麦抗逆机制，采用电子克隆结合RT-PCR的方法，从小麦叶片中分离出 TaSABP2基因；利用生物信息学手段分析其序列特征；运用实时荧光定量反转录PCR（qRT-PCR）技术检测其在不同条件下的表达情况。结果表明，小麦 TaSABP2基因的cDNA全长为878 bp，包含一个长度为807 bp的开放阅读框，编码268个氨基酸。 TaSABP2基因的编码蛋白包含Abhydrolase及PLN02211两个结构域及一个酶活性中心（VVLVGHSLGG），属于Abhydrolase超家族的成员。其蛋白二级结构由四种形式构成，包括40.3%的α-螺旋、16.42%的延伸链、4.48%的β转角、38.81%的无规则卷曲。通过与其他植物的氨基酸序列进行多重序列比对，发现功能区域的氨基酸序列较为保守。qRT-PCR结果表明， TaSABP2基因的表达具有较强的组织特异性，植物激素ABA和BR处理及干旱和盐胁迫处理，均能诱导该基因的表达量迅速增加。以上结果表明， TaSABP2基因在小麦抵抗逆境胁迫过程中起到一定作用。 |
| 英文摘要: |
| The impact of abiotic stresses on wheat production is serious. To explore stress resistance genes in wheat,and to clearify the mechanism of their regulation,and to cultivate new varieties are important for wheat production. The aim of the study was to clone TaSABP2 gene in wheat,and to analyze its sequence characteristic and expression pattern,in order to further clarify the wheat resistance mechanism. The full sequence of TaSABP2 was cloned. The sequence characteristics were analyzed with network resources and its expression pattern under different conditions was detected by real-time quantitative PCR. The results showed that the full-length sequence of TaSABP2 consists of 878 bp with an intact open reading frame,encoding a polypeptide of 268 amino acids. TaSABP2 contains two functional domains of Abhydrolase and PLN02211,and an enzyme active site sequence (VVLVGHSLGG),and it is a member of Abhydrolase superfamily. The proportion of secondary structures of TaSABP2 are 40.3% for α-helix,16.42% for β-sheet,4.48% for β-turn and 38.81% for random coil. The multiple sequence alignment based on the deduced amino acid sequences of TaSABP2 and other PALs of nine species showed the functional region is conserved. qRT-PCR analysis revealed that TaSABP2 gene expressed variously in different tissues. The expression of the gene was increased rapidly under foliar spraying of ABA and BR and under drought and salt stress treatments. TaSABP2 gene may have a certain effect on the stress resistance. |
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