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作者	单位
毕惠惠，毛伟伟，李海霞，程西永，周渭皓，周思远，武会芳，许海霞	（河南农业大学/省部共建小麦玉米作物学国家重点实验室,河南郑州 450046）

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中文摘要:

小麦AKT1具有钾离子转运功能。为了给小麦钾离子吸收转运机制的研究提供参考，本研究从普通小麦品种石4185中扩增得到TaAKT1基因，其编码区序列全长2 694 bp，生物信息学分析表明，TaAKT1蛋白包含897个氨基酸，相对分子质量为100.92 kDa，具有钾离子通道蛋白的特征序列TxxTxGYGD。多序列比对发现，TaAKT1蛋白与大麦HvAKT1蛋白的进化关系最近，相似性达97.22%。利用qRT-PCR分析发现，在正常条件下TaAKT1基因在小麦根部表达量约为叶片中表达量的5倍，低钾环境下TaAKT1的表达量在小麦根部明显上调，叶部的表达量无明显变化。利用酵母功能互补实验发现，转TaAKT1基因的酵母在含有5 mmol·L^-1 KCl的AP培养基上正常生长，而转空载体的酵母长势受到抑制，说明TaAKT1具有钾离子转运功能。同时转TaAKT1基因与 TaCIPK23基因的酵母能在含60 μmol·L^-1 KCl的AP培养基上生长，而转空载体与转TaAKT1基因的酵母菌株均不能生长，说明TaCIPK23可以增强TaAKT1的钾离子吸收能力。

英文摘要:

Potassium is an essential macroelement for the growth and development of plants. Cloning and functional analysis of wheat AKT1 genes can provide theoretical basis for the study of potassium ion absorption mechanism in wheat. In this study, we cloned a TaAKT1 gene from wheat cultivar Shi4185. Bioinformatics analyses showed that the cDNA of TaAKT1 is 2 694 bp in length and encodes a protein of 897 amino acids with a molecular mass of 100.92 kDa and a characteristic sequence TxxTxGYGD of potassium channel protein. Multiple sequence analysis found that TaAKT1 and HvAKT1(Hordeum vulgare) had the most similarity of 97.22%. Real-time PCR analysis showed that the expression level of TaAKT1 gene in wheat root was about 5 times that of leaf. Under a low-potassium condition, the expression level of TaAKT1 gene in root was strongly up-regulated, but that in leaf was not changed drastically. Functional complementation in yeast revealed that WΔ3 strain transformed with TaAKT1 could grow normally on the AP medium with 5 mmol·L^-1 KCl, while the growth of WΔ3 transformed with empty vector (pYPGE15) was significantly inhibited,suggesting that TaAKT1 plays a role in K⁺ uptake. The WΔ3 strain transformed with both TaAKT1 and TaCIPK23 could grow on the AP medium supplied with 60 μmol·L^-1 KCl, while the strains with empty vector or with TaAKT1 can not survive. The result suggested that the function of TaAKT1 could be enhanced by TaCIPK23. Thus, we concluded that TaAKT1 gene might serve as a potassium uptake channel protein and it might be regulated by TaCIPK23.

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